Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.)
Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai (Brassica Campestris L.)
复制标题
比较蛋白质组学揭示了五彩（Brassica Campestris L.）中碳水化合物和氨基酸代谢增强的冷驯化机制
DOI：
10.3390/plants8110474
复制
发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Wang Chenggang
中科院分区：
文献类型：
--
作者：
Yuan Lingyun;Xie Shilei;Nie Libing;Zheng Yushan;Wang Jie;Huang Ju;Zhao Mengru;Zhu Shidong;Hou Jinfeng;Chen Guohu;Wang Chenggang
不结球白菜在低温下的冷驯化研究资料有限。在这项研究中，同量异位素标签相对和绝对定量（iTRAQ）被用来说明冷驯化的分子机制。与对照相比，共鉴定出89个差异表达蛋白。在这些蛋白质中，35个蛋白质表达上调（54个蛋白质表达下调）。这些差异表达的蛋白质被分类为在碳水化合物代谢、光合作用和能量代谢、氧化防御、氨基酸代谢、代谢进程、冷调节、甲基化进程和信号转导中起作用。果糖、葡萄糖和蔗糖在冷驯化过程中显著增加。首次报道了低温下天冬氨酸、丝氨酸、谷氨酸、脯氨酸和苏氨酸的显著积累。对9个DEPs进行实时荧光定量PCR分析，结果表明，6个基因的转录表达模式与其蛋白表达丰度一致。我们的研究结果表明，五彩通过调控几个关键蛋白的表达来适应低温。此外，碳水化合物和氨基酸的转化在提高五彩冷同化中起着不可或缺的重要作用。
Limited information is available on the cold acclimation of non-heading Chinese cabbage (NHCC) under low temperatures. In this study, the isobaric tags for relative and absolute quantification (iTRAQ) were used to illustrate the molecular machinery of cold acclimation. Compared to the control (Cont), altogether, 89 differentially expressed proteins (DEPs) were identified in wucai leaves responding to low temperatures (LT). Among these proteins, 35 proteins were up-regulated ((and 54 were down-regulated). These differentially expressed proteins were categorized as having roles in carbohydrate metabolism, photosynthesis and energy metabolism, oxidative defense, amino acid metabolism, metabolic progress, cold regulation, methylation progress, and signal transduction. The fructose, glucose, and sucrose were dramatically increased in response to cold acclimation. It was firstly reported that aspartate, serine, glutamate, proline, and threonine were significantly accumulated under low temperatures. Results of quantitative real-time PCR analysis of nine DEPs displayed that the transcriptional expression patterns of six genes were consistent with their protein expression abundance. Our results demonstrated that wucai acclimated to low temperatures through regulating the expression of several crucial proteins. Additionally, carbohydrate and amino acid conversion played indispensable and vital roles in improving cold assimilation in wucai.
登录
查看更多内容
DOI：
10.1111/tpj.12766
发表时间：
2015-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Snowden CJ;Thomas B;Baxter CJ;Smith JA;Sweetlove LJ
通讯作者：
Sweetlove LJ
影响因子：
2.1
作者：
Baohua Wang;R. Fu;Mi Zhang;Zhenqian Ding;Lei Chang;Xinyu Zhu;Yafeng Wang;Baoxiang Fan;
通讯作者：
Baohua Wang;R. Fu;Mi Zhang;Zhenqian Ding;Lei Chang;Xinyu Zhu;Yafeng Wang;Baoxiang Fan;
影响因子：
3.3
作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
通讯作者：
Lintong Yang;Y. Qi;Yi-Bin Lu;Peng Guo;W. Sang;H. Feng;Hong-Xing Zhang;Lisong Chen
高温对不结球白菜耐热和感热基因型光合作用、叶绿体抗氧化能力及碳同化能力的影响
影响因子：
1.9
作者：
Yuan Lingyun;Yuan Yujie;Liu Shan;Wang Jie;Zhu Shidong;Chen Guohu;Hou Jinfeng;Wang Chenggang
通讯作者：
Wang Chenggang
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
Reto J Strassert;Alaka Srivastava 'and Govindjee
通讯作者：
Reto J Strassert;Alaka Srivastava 'and Govindjee