Low-energy shock wave therapy ameliorates ischemic-induced overactive bladder in a rat model.
Low-energy shock wave therapy ameliorates ischemic-induced overactive bladder in a rat model.
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DOI：
10.1038/s41598-022-26292-x
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发表时间：
2022-12-19
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究旨在探讨低能量冲击波治疗（LESW）对缺血性膀胱过度活动症大鼠的治疗作用及其机制。16周龄雄性SD大鼠分为3组：动脉损伤组（AI）、AI + LESW组（AI-SW）和对照组。LESW在20-23周龄期间在AI-SW中照射。在24周龄时，进行有意识的膀胱测压（每个n = 8）。AI组的排尿间隔（平均值± SEM：5.1 ± 0.8 min）比对照组（17.3 ± 3.0 min）缩短，而AI-SW组（14.9 ± 3.3 min）观察到显著改善。AI-SW组膀胱血流量较AI组明显增加。基因芯片分析显示，AI-SW组膀胱可溶性鸟苷酸环化酶（sGC）α1和β1基因表达高于AI组。sGCα1和sGCβ1蛋白表达在AI-SW组和对照组均高于AI组。环磷酸鸟苷（cGMP）在AI-SW中升高。作为早期遗传反应，血管内皮生长因子和CD 31在首次LESW后24 h高度表达。在AI中观察到的上皮下变薄在AI-SW中得到恢复。sGC-cGMP的激活可能在LESW的膀胱功能恢复中发挥治疗作用。
This study was to evaluate whether Low-energy shock wave therapy (LESW) improves ischemic-induced overactive bladder in rats and investigate its therapeutic mechanisms. Sixteen-week-old male Sprague–Dawley rats were divided into three groups: arterial injury (AI), AI with LESW (AI-SW), and control groups. LESW was irradiated in AI-SW during 20–23 weeks of age. At 24 weeks of age, conscious cystometry was performed (each n = 8). The voiding interval was shortened in AI (mean ± SEM: 5.1 ± 0.8 min) than in control (17.3 ± 3.0 min), whereas significant improvements were observed in AI-SW (14.9 ± 3.3 min). The bladder blood flow was significantly increased in AI-SW than in AI. Microarray analysis revealed higher gene expression of soluble guanylate cyclase (sGC) α1 and β1 in the bladder of AI-SW compared to AI. Protein expression of sGCα1 and sGCβ1 was higher in AI-SW and control groups than in AI. Cyclic guanosine monophosphate (cGMP) was elevated in AI-SW. As an early genetic response, vascular endothelial growth factor and CD31 were highly expressed 24 h after the first LESW. Suburothelial thinning observed in AI was restored in AI-SW. Activation of sGC-cGMP may play a therapeutic role of LESW in the functional recovery of the bladder.
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影响因子：
3.3
作者：
Serizawa, Fukashi;Ito, Kenta;Satomi, Susumu
通讯作者：
Satomi, Susumu
影响因子：
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通讯作者：
Yamaguchi, Osamu
影响因子：
1.3
作者：
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通讯作者：
Ito, Akihiro