Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils.
Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils.
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从甘蔗中分离的内生真菌中间脉孢菌 DP8-1 对敌草隆的生物降解及其修复敌草隆污染土壤的潜力
DOI：
10.1371/journal.pone.0182556
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zeng D
中科院分区：
文献类型：
--
作者：
Wang Y;Li H;Feng G;Du L;Zeng D
本研究从甘蔗根中分离到一株降解二脲的内生菌DP8-1。根据形态特征和序列分析，内生菌为中间神经孢子菌。研究了温度、pH、接种量、碳源、初始浓度等发酵参数对降解效率的影响。结果表明，在最佳降解条件下，菌株DP8-1在3 d内可降解99%的迪乌隆。降解谱研究表明，菌株DP8-1还能降解并利用吡脲、莫脲、异丙脲、氯脲、利脲和氯脲作为菌株生长的底物。在液相色谱-质谱分析的基础上，菌株DP8-1通过连续的N-脱烷基反应，代谢二恶龙生成N-（3,4-二氯苯）-尿素和N-（3,4-二氯苯）-N-甲基脲。在一项土壤生物强化试验中，将菌株DP8-1接种于处理过的土壤中，可有效提高土壤中diuron的消失率。
A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron.
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影响因子：
2.8
作者：
BUNYARD, BA;NICHOLSON, MS;ROYSE, DJ
通讯作者：
ROYSE, DJ
影响因子：
4.1
作者：
El Sebai, Talaat;Devers, Marion;Martin-Laurent, Fabrice
通讯作者：
Martin-Laurent, Fabrice
影响因子：
3.6
作者：
Ellegaard-Jensen, Lea;Aamand, Jens;Rosendahl, Soren
通讯作者：
Rosendahl, Soren
影响因子：
4.1
作者：
Nygren, Kristiina;Strandberg, Rebecka;Johannesson, Hanna
通讯作者：
Johannesson, Hanna
乙醇和蛋白质的乙醇植物副产品使用可食用的真菌神经孢子中间和曲曲霉。
影响因子：
--
作者：
Bátori V;Ferreira JA;Taherzadeh MJ;Lennartsson PR
通讯作者：
Lennartsson PR