Detection of microbial translocation in HIV and SIV infection using the Limulus amebocyte lysate assay is masked by serum and plasma.
Detection of microbial translocation in HIV and SIV infection using the Limulus amebocyte lysate assay is masked by serum and plasma.
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DOI：
10.1371/journal.pone.0041258
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
NWCS 319 and ACTG 5175 study team
中科院分区：
文献类型：
--
作者：
Balagopal A;Gama L;Franco V;Russell JN;Quinn J;Higgins Y;Smeaton LM;Clements JE;Thomas DL;Gupta A;NWCS 319 and ACTG 5175 study team
微生物易位 (MT) 被认为是 HIV 相关免疫激活发病机制的主要贡献者，而来自革兰氏阴性细菌的循环脂多糖 (LPS) 是该过程的主要测量指标。然而，相关研究因LPS检测结果不一致而受到阻碍。样本取自参加 PEARLS 研究 (ACTG A5175) 的 HIV 感染成年人和参加使用 MRI 弹性成像进行肝病分期研究的 HIV-HCV 共同感染参与者。猪尾猕猴标本取自 SIV 感染和未感染的动物。使用 LAL 测定法对样品进行 LPS 检测，并进行重氮偶联修饰以提高检测灵敏度。当将外源性 LPS 添加到猕猴血浆中时，与对照相比，在 20% 血浆浓度的 10/10 (100%) 样品中发现 LPS 检测受到 >25% 的抑制；相比之下，5/10 (50%) 样品在 2% 血浆浓度 (p = 0.07) 和 0/10 (0%) 在 0.1% 血浆浓度 (p = 0.004) 时显示出 > 25% 的 LPS 检测抑制。同样，当将 LPS 添加到人血清中时，与对照相比，2% 血清浓度的样品中有 5/12 (42%) 的 LPS 检测受到 >25% 的抑制，而 0.1% 血清中的 0/12 (0%) 的样品显示出 >25% 的 LPS 检测抑制 (p = 0.07)。同样，通过稀释，在不含外源 LPS 的人体血清中的 LPS 检测得到改善：2% 血清中的 2/12 (17%) 人体样本中检测到 LPS，范围为 3,436–4,736 pg/mL，而 0.1% 血清中的 LPS 检测率为 9/12 (75%)，范围为 123 pg/mL –60,131 pg/mL (p = 0.016)。在同一天两个不同时间采样的 HIV-HCV 共同感染参与者的单独验证队列中，在 0.2% 血浆中测量的 LPS 和重氮偶联在第一个和第二个样本之间密切相关 (R = 0.66，p<0.05)。未稀释的血清和血浆掩膜LPS检测。 MT 的范围可能被大大低估。
Microbial translocation (MT) is thought to be a major contributor to the pathogenesis of HIV-related immune activation, and circulating lipopolysaccharide (LPS) from Gram-negative bacteria is the principle measurement of this process. However, related research has been impeded by inconsistent LPS test results. Specimens were obtained from HIV-infected adults enrolled in the PEARLS study (ACTG A5175) and HIV-HCV co-infected participants enrolled in a study of liver disease staging using MRI elastography. Pig-tailed macaque specimens were obtained from SIV-infected and –uninfected animals. Samples were tested for LPS using the LAL assay with diazo-coupling modifications to improve sensitive detection. When exogenous LPS was added to macaque plasma, >25% inhibition of LPS detection was found in 10/10 (100%) samples at 20% plasma concentration compared to control; in contrast 5/10 (50%) samples at 2% plasma concentration (p = 0.07) and 0/10 (0%) at 0.1% plasma concentration (p = 0.004) showed >25% inhibition of LPS detection. Similarly, when LPS was added to human serum, >25% inhibition of LPS detection was found in 5/12 (42%) of samples at 2% serum concentration compared to control, while 0/12 (0%) of samples in 0.1% serum showed >25% inhibition of LPS detection (p = 0.07). Likewise, LPS detection in human sera without exogenous LPS was improved by dilution: LPS was detected in 2/12 (17%) human samples in 2% serum, ranging from 3,436–4,736 pg/mL, compared to 9/12 (75%) samples in 0.1% serum, ranging from 123 pg/mL –60,131 pg/mL (p = 0.016). In a separate validation cohort of HIV-HCV co-infected participants sampled at two different times on the same day, LPS measured in 0.2% plasma and with diazo-coupling was closely correlated between the first and second samples (R = 0.66, p<0.05). Undiluted serum and plasma mask LPS detection. The extent of MT may be substantially underestimated.
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影响因子：
4.1
作者：
Jerala, Roman
通讯作者：
Jerala, Roman
影响因子：
3.4
作者：
HURLEY, JC;TOSOLINI, FA;LOUIS, WJ
通讯作者：
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DOI：
10.1097/qai.0b013e3181e0cdea
发表时间：
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期刊：
Journal of acquired immune deficiency syndromes (1999)
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作者：
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作者：
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通讯作者：
Ray, Stuart C.
DOI：
10.1073/pnas.0901983106
发表时间：
2009-04-21
影响因子：
11.1
作者：
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通讯作者：
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