The biology and methodology of assisted reproduction in deer mice (Peromyscus maniculatus).
The biology and methodology of assisted reproduction in deer mice (Peromyscus maniculatus).
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鹿小鼠辅助生殖的生物学和方法论（peromyscus maniculatus）。
DOI：
10.1016/j.theriogenology.2011.07.044
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发表时间：
2012-01-15
期刊：
影响因子：
2.8
通讯作者：
Szalai G
中科院分区：
文献类型：
--
作者：
Veres M;Duselis AR;Graft A;Pryor W;Crossland J;Vrana PB;Szalai G
Although laboratory-reared species of the genus Peromyscus - including deer mice - are used as model animals in a wide range of research, routine manipulation of Peromyscus embryogenesis and reproduction has been lagging. The objective of the present study was to optimize conditions for oocyte/embryo retrieval and for in vitro culturing. On average, 6.4 oocytes per mouse were recovered when two doses of 15 IU of PMSG was given 24 h apart, followed by 15 IU of hCG 48 h later. Following this hormone priming, females mated overnight with a fertile male yielded an average of 9.1 two-cell stage embryos. Although two-cell stage embryos developed to 8-cell stage in KSOM media in vitro, but not further, embryos recovered at the 8- to 16-cell stages developed into fully expanded blastocysts when cultured in M16 media in vitro. These blastocysts had full potential to develop into late stage fetuses and possibly into live pups. As a result of the present work, all stages of Peromyscus pre-implantation development are now obtainable in numbers sufficient for molecular or other analyses. These advances provide the opportunity for routine studies involving embryo transfer (e.g. chimeras, transgenics), and preservation of genetic lines by cryopreservation.
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影响因子：
3.7
作者：
Ching Ouyang;Smith, David D.;Krontiris, Theodore G.
通讯作者：
Krontiris, Theodore G.
影响因子：
--
作者：
KLEIN, MS;MARKERT, CL
通讯作者：
MARKERT, CL
影响因子：
1.7
作者：
ROTH, VL;KLEIN, MS
通讯作者：
KLEIN, MS
影响因子：
6.1
作者：
Nargund, G;Waterstone, J;Campbell, S
通讯作者：
Campbell, S
DOI：
10.1530/jrf.0.0720009
发表时间：
1984-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
LEESE, HJ;BARTON, AM
通讯作者：
BARTON, AM