Genomic differences between sequence types 1 and 104 of Streptococcus suis Serotype 2.
Genomic differences between sequence types 1 and 104 of Streptococcus suis Serotype 2.
复制标题
作者：
Kerdsin A;Takeuchi D;Akeda Y;Nakamura S;Gottschalk M;Oishi K
猪链球菌是一种人畜共患病病原体，与受感染的猪或受污染的猪肉制品有密切接触的人会受到侵袭性感染。猪链球菌血清2型序列类型(ST)1菌株大多与脑膜炎有关，而ST104菌株大多是从人类败血症病例中恢复的。目前还没有在基因组水平上比较ST1和ST104菌株的数据，特别是关于毒力相关基因的数据。因此，本研究对这两种STS进行了基因组比较。从脑膜炎患者脑脊液中分离的ST1株(ID26154)和从败血症患者血液中分离的ST104株(ID24525)分别用454GS Junior系统进行了鸟枪式焦磷酸测序。利用Artemis比较工具(ACT)对ST1分离物和ST104分离物进行基因组比较，以确定ST1和ST104分离物的差异区域(RDS)。58个RDS是ST104基因组所特有的，主要涉及新陈代谢和细胞功能活性、细胞壁锚定蛋白、噬菌体和移动遗传元件、ABC型转运蛋白、双组分信号转导和抗生素蛋白。一些毒力基因在ST104基因组中也存在，这些基因大多存在于ST1菌株中。全基因组比较是鉴定猪链球菌2型不同STS之间基因组区域差异的有力工具，有助于鉴定参与感染发病机制的毒力的分子基础。
Streptococcus suis is a zoonotic pathogen that can cause invasive infections in humans who are in close contact with infected pigs or contaminated pork-derived products. S. suis serotype 2 sequence type (ST) 1 strains are mostly associated with meningitis, whereas ST104 strains are mostly recovered from sepsis cases in humans. No data are available for comparison of the ST1 and ST104 strains at the genomic level, particularly concerning virulence-associated genes. Thus, genomic comparison of both STs was performed in this study. An ST1 isolate (ID26154) from the cerebrospinal fluid of a patient with meningitis and an ST104 isolate (ID24525) from the blood of a patient with sepsis were subjected to shotgun pyrosequencing using the 454 GS Junior System. Genomic comparison was conducted between the ST1 isolate and the ST104 isolate using the Artemis Comparison Tool (ACT) to identify the region of differences (RDs) between ST1 and ST104. Fifty-eight RDs were unique to the ST104 genome and were mainly involved in metabolism and cell functional activities, cell wall anchored proteins, bacteriophages and mobile genetic elements, ABC-type transporters, two-component signal transductions, and lantibiotic proteins. Some virulence genes mostly found in ST1 strains were also present in the ST104 genome. Whole-genome comparison is a powerful tool for identifying genomic region differences between different STs of S. suis serotype 2, leading to the identification of the molecular basis of virulence involved in the pathogenesis of the infection.
登录
查看更多内容
影响因子：
3.3
作者：
Silva, Luciana M. G.;Baums, Christoph G.;Valentin-Weigand, Peter
通讯作者：
Valentin-Weigand, Peter
影响因子：
3.6
作者：
Manetti, Andrea G. O.;Zingaretti, Chiara;Margarit, Immaculada
通讯作者：
Margarit, Immaculada
DOI：
10.3390/pathogens5030045
发表时间：
2016-06-24
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Okura M;Osaki M;Nomoto R;Arai S;Osawa R;Sekizaki T;Takamatsu D
通讯作者：
Takamatsu D
影响因子：
4.4
作者：
Estrada AA;Gottschalk M;Gebhart CJ;Marthaler DG
通讯作者：
Marthaler DG
影响因子：
3.3
作者：
Takamatsu, Daisuke;Nishino, Hiroto;Sekizaki, Tsutomu
通讯作者：
Sekizaki, Tsutomu