Low expression of miR-19a-5p is associated with high mRNA expression of diacylglycerol O-acyltransferase 2 (DGAT2) in hybrid tilapia.
Low expression of miR-19a-5p is associated with high mRNA expression of diacylglycerol O-acyltransferase 2 (DGAT2) in hybrid tilapia.
复制标题
杂交罗非鱼中 miR-19a-5p 的低表达与二酰基甘油 O-酰基转移酶 2 (DGAT2) 的高 mRNA 表达相关。
DOI：
10.1016/j.ygeno.2021.05.016
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发表时间：
2021-05
期刊：
影响因子：
4.4
通讯作者：
Zhou Luojing
中科院分区：
文献类型：
--
作者：
Zhong Huan;Zhou Yi;Xu Qian;Yan Jinpeng;Zhang Xiaojin;Zhang Hong;Tang Zhanyang;Xiao Jun;Guo Zhongbao;Luo Yongju;Zhou Luojing
DGAT2(酰基辅酶A：二酰甘油酰基转移酶2)是催化甘油三酯(TG)合成的关键限速酶。本研究以尼罗罗非鱼(♀)和蓝罗非鱼(♂)为亲本，通过杂交获得了杂交罗非鱼。测定了这些罗非鱼肝脏中的甘油三酯含量。结果表明，杂交罗非鱼的甘油三酯含量较高。此外，还测定了罗非鱼肝脏中蛋白质和mRNA的表达水平。杂交鱼肝脏中DGAT2的mRNA和蛋白表达水平较高。用HEK293T细胞进行的荧光素酶报告分析表明，miRNA-19a-5p针对DGAT2的3‘UTR，这表明了一种直接的调控机制。利用qRT-PCR技术，我们发现尼罗罗非鱼和杂交罗非鱼中DGAT2基因的表达水平与miRNA-19a-5p的表达水平呈负相关。综上所述，这些发现提供了证据，表明miRNA-19a-5p参与了罗非鱼脂肪代谢调节中的甘油三酯合成。
DGAT2 (acyl CoA:diacylglycerol acyltransferase 2) is a key and rate-limiting enzyme that catalyzes the final step of triglyceride (TG) synthesis. In this study, hybrid tilapia were generated from Nile tilapia (♀) and blue tilapia (♂) crossing. The TG content levels in the liver of these tilapia were measured. The results showed that the TG content was higher in the hybrid tilapia. In addition, protein and mRNA expression levels in the tilapia livers were determined. Higher hepatic mRNA and protein expression of DGAT2 in the hybrid fish was found. A luciferase reporter assay with HEK293T cells revealed that miRNA-19a-5p targeted the 3′UTR of DGAT2, suggesting a direct regulatory mechanism. Using qRT-PCR, we found that DGAT2 mRNA levels had a negative correlation with miRNA-19a-5p expression in Nile tilapia and hybrid. Taken together, these findings provide evidence that miRNA-19a-5p is involved in TG synthesis in the regulation of lipid metabolism in tilapia.
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