Quantifying the cellular uptake of antibody-conjugated Au nanocages by two-photon microscopy and inductively coupled plasma mass spectrometry.
Quantifying the cellular uptake of antibody-conjugated Au nanocages by two-photon microscopy and inductively coupled plasma mass spectrometry.
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DOI：
10.1021/nn901392m
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发表时间：
2010-01-26
期刊：
影响因子：
17.1
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Au L;Zhang Q;Cobley CM;Gidding M;Schwartz AG;Chen J;Xia Y
金纳米笼的局部表面等离子体共振峰在近红外区域表现出广泛的双光子光致发光带从450至650 nm时，由钛：蓝宝石激光在800 nm激发。明亮的发光使得探索使用Au纳米笼作为双光子显微镜的一类新型光学成像剂成为可能。在这项工作中，我们已经证明了使用双光子显微镜作为一种方便的工具，直接检查U87 MGwtEGFR细胞的抗体结合和PEG化Au纳米笼的摄取。我们还将双光子显微镜的结果与电感耦合等离子体质谱法获得的数据相关联。结合在一起，这些结果表明，抗体缀合的Au纳米笼通过抗体-抗原结合附着到细胞表面，然后通过受体介导的内吞作用内化到细胞中。细胞摄取过程取决于许多参数，包括孵育时间、孵育温度、Au纳米笼的尺寸和固定在每个纳米笼上的抗体的数量。
Gold nanocages with localized surface plasmon resonance peaks in the near-infrared region exhibited a broad two-photon photoluminescence band extending from 450 to 650 nm when excited by a Ti:sapphire laser at 800 nm. The bright luminescence makes it possible to explore the use of Au nanocages as a new class of optical imaging agents for two-photon microscopy. In this work, we have demonstrated the use of two-photon microscopy as a convenient tool to directly examine the uptake of antibody-conjugated and PEGylated Au nanocages by U87MGwtEGFR cells. We have also correlated the results from two-photon microscopy with the data obtained by inductively coupled plasma mass spectrometry. Combined together, these results indicate that the antibody-conjugated Au nanocages were attached to the surface of the cells through antibody-antigen binding and then internalized into the cells via receptor-mediated endocytosis. The cellular uptake process was dependent on a number of parameters, including incubation time, incubation temperature, size of the Au nanocages, and the number of antibodies immobilized on each nanocage.
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影响因子：
15
作者：
Imura, K;Nagahara, T;Okamoto, H
通讯作者：
Okamoto, H
影响因子：
10.8
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
38.3
作者：
Jiang, Wen;Kim, Betty Y. S.;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
10.8
作者：
Wang, YW;Xie, XY;Wang, LV
通讯作者：
Wang, LV