Modeling of protein electrophoresis in silica colloidal crystals having brush layers of polyacrylamide.
Modeling of protein electrophoresis in silica colloidal crystals having brush layers of polyacrylamide.
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DOI：
10.1002/elps.201200413
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发表时间：
2013-03
期刊：
影响因子：
2.9
通讯作者：
Wirth, Mary J.
中科院分区：
文献类型：
--
作者：
Birdsall, Robert E.;Koshel, Brooke M.;Hua, Yimin;Ratnayaka, Saliya N.;Wirth, Mary J.
在二氧化硅胶体晶体中筛选mm尺寸的蛋白质的特征在于标称350和500 nm的粒径，其中胶体晶体用聚丙烯酰胺的刷层进行化学改性。一个模型的开发，涉及减少电泳迁移率的实验测量的孔隙度。该模型拟合的数据，没有可调参数的情况下，二氧化硅胶体晶体包装在毛细管中，独立的测量的孔隙半径从流量数据。该模型还适合在一个高度有序的胶体晶体中形成的通道，其中未知的孔半径作为拟合参数的电泳数据。小至0.4 μm的板高表明了小型化分离的潜力。谱带增宽随着孔半径接近蛋白质半径而增加，表明对增宽的主要贡献是孔半径的空间异质性。结果定量支持的概念，筛分发生在二氧化硅胶体晶体中的蛋白质，并有利于设计新的分离，将受益于小型化。
Sieving of proteins in silica colloidal crystals of mm dimensions is characterized for particle diameters of nominally 350 and 500 nm, where the colloidal crystals are chemically modified with a brush layer of polyacrylamide. A model is developed that relates the reduced electrophoretic mobility to the experimentally measurable porosity. The model fits the data with no adjustable parameters for the case of silica colloidal crystals packed in capillaries, for which independent measurements of the pore radii were made from flow data. The model also fits the data for electrophoresis in a highly ordered colloidal crystal formed in a channel, where the unknown pore radius was used as a fitting parameter. Plate heights as small as 0.4 μm point to the potential for miniaturized separations. Band broadening increases as the pore radius approaches the protein radius, indicating that the main contribution to broadening is the spatial heterogeneity of the pore radius. The results quantitatively support the notion that sieving occurs for proteins in silica colloidal crystals, and facilitate design of new separations that would benefit from miniaturization.
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影响因子：
7.4
作者：
Wei, Bingchuan;Malkin, Douglas S.;Wirth, Mary J.
通讯作者：
Wirth, Mary J.
DOI：
10.1016/s0021-9673(01)85026-3
发表时间：
1987-06-26
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Wirth, Mary J.
影响因子：
5.5
作者：
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通讯作者：
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影响因子：
4.1
作者：
Cabooter, Deirdre;Billen, Jeroen;Desmet, Gert
通讯作者：
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