Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain.
Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain.
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DOI：
10.1002/cne.22543
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发表时间：
2011-03-01
影响因子：
2.5
通讯作者：
Okano, Hideyuki
中科院分区：
文献类型：
--
作者：
Sawamoto, Kazunobu;Hirota, Yuki;Alfaro-Cervello, Clara;Soriano-Navarro, Mario;He, Xiaoping;Hayakawa-Yano, Yoshika;Yamada, Masayuki;Hikishima, Keigo;Tabata, Hidenori;Iwanami, Akio;Nakajima, Kazunori;Toyama, Yoshiaki;Itoh, Toshio;Alvarez-Buylla, Arturo;Manuel Garcia-Verdugo, Jose;Okano, Hideyuki
成体侧脑室室管膜下区（SVZ）含有神经干细胞。在啮齿类动物中，这些细胞产生成神经细胞，成神经细胞沿着吻侧迁移流（RMS）向嗅球沿着迁移。心室壁的神经干细胞龛在包括灵长类在内的各种动物中是保守的。然而，目前还不清楚非人灵长类动物的SVZ和RMS组织与啮齿类动物和人类的SVZ和RMS组织有何关系。在这里，我们研究了SVZ和RMS的成年和新生儿普通绒猴（Callithrix jacchus），一种新的世界灵长类动物广泛用于神经科学，通过电子显微镜，免疫组化检测细胞类型特异性标记。绒猴SVZ含有与啮齿动物SVZ的B、C和A型细胞在其标记物表达和形态上相似的细胞。成年绒猴SVZ有一个三层组织，在人类大脑中，室管膜层，低细胞层和星形胶质细胞带层。然而，在成人前部和新生儿SVZ中，低细胞层非常薄或缺失。抗PSA-NCAM染色的前SVZ在整个安装心室壁制备的成年绒猴揭示了一个广泛的网络细长的细胞聚集体类似于啮齿动物的神经母细胞链。在Matrigel中培养的绒猴SVZ外植体的延时记录显示成神经细胞像啮齿动物A型细胞一样成链迁移。这些结果表明，在SVZ的神经发生和神经元迁移的一些功能是共同的绒猴，人类和啮齿动物。对成年和新生期绒猴SVZ的基本描述将有助于进一步研究灵长类动物的成年神经发生。
The adult subventricular zone (SVZ) of the lateral ventricle contains neural stem cells. In rodents, these cells generate neuroblasts that migrate as chains toward the olfactory bulb along the rostral migratory stream (RMS). The neural-stem-cell niche at the ventricular wall is conserved in various animal species, including primates. However, it is unclear how the SVZ and RMS organization in nonhuman primates relates to that of rodents and humans. Here we studied the SVZ and RMS of the adult and neonatal common marmoset (Callithrix jacchus), a New World primate used widely in neuroscience, by electron microscopy, and immunohistochemical detection of cell-type-specific markers. The marmoset SVZ contained cells similar to type B, C, and A cells of the rodent SVZ in their marker expression and morphology. The adult marmoset SVZ had a three-layer organization, as in the human brain, with ependymal, hypocellular, and astro-cyte-ribbon layers. However, the hypocellular layer was very thin or absent in the adult-anterior and neonatal SVZ. Anti-PSA-NCAM staining of the anterior SVZ in whole-mount ventricular wall preparations of adult marmosets revealed an extensive network of elongated cell aggregates similar to the neuroblast chains in rodents. Time-lapse recordings of marmoset SVZ explants cultured in Matrigel showed the neuroblasts migrating in chains, like rodent type A cells. These results suggest that some features of neurogenesis and neuronal migration in the SVZ are common to marmosets, humans, and rodents. This basic description of the adult and neonatal marmoset SVZ will be useful for future studies on adult neurogenesis in primates.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
DOI：
10.1073/pnas.96.10.5768
发表时间：
1999-05-11
影响因子：
11.1
作者：
Kornack, DR;Rakic, P
通讯作者：
Rakic, P
影响因子：
6.2
作者：
Danilov, Alexandre I.;Gomes-Leal, Walace;Lindvall, Olle
通讯作者：
Lindvall, Olle
影响因子：
2.5
作者：
Brown, JP;Couillard-Després, S;Kuhn, HG
通讯作者：
Kuhn, HG
影响因子：
5.3
作者：
Kohwi, M;Osumi, N;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A