Bacterial contamination of frequently touched objects in a tertiary care hospital of Pokhara, Nepal: how safe are our hands?
Bacterial contamination of frequently touched objects in a tertiary care hospital of Pokhara, Nepal: how safe are our hands?
复制标题
DOI：
10.1186/s13756-018-0385-2
复制
发表时间：
2018
影响因子：
5.5
通讯作者：
Gokhale S
中科院分区：
文献类型：
--
作者：
Bhatta DR;Hamal D;Shrestha R;Hosuru Subramanya S;Baral N;Singh RK;Nayak N;Gokhale S
医院中患者和医护人员经常接触的物体含有潜在的病原体，可能成为传染源。本研究旨在确定患者、访客和医护人员经常接触的常见医院物品的细菌污染情况。从生物识别考勤设备表面、电梯按钮、门把手、楼梯栏杆、电话机和水龙头等不同地点总共收集了 232 个样本。通过标准微生物学技术对分离株进行分离、鉴定和抗生素敏感性测试。通过微量滴定板方法测试金黄色葡萄球菌分离株的生物膜形成能力。共采集样品232份，从181份样品中分离出219株细菌。金黄色葡萄球菌是最常见的细菌分离株 (44/219)。大多数金黄色葡萄球菌分离株是从电梯按钮、生物识别考勤设备和门把手中回收的。在金黄色葡萄球菌分离株中，36.3%（16/44）为耐甲氧西林金黄色葡萄球菌（MRSA），其余为甲氧西林敏感金黄色葡萄球菌（MSSA）。在 44 株金黄色葡萄球菌分离株中，12 株（29.5%）具有多重耐药性，14 株（31.8%）产生生物膜。大多数 MRSA 分离株（62.5% (10/16)）是生物膜产生者。不动杆菌是最常见的革兰氏阴性菌株，其次是大肠杆菌和假单胞菌。经常接触的物体被检测到存在多种潜在病原体和正常菌群的高度细菌污染。金黄色葡萄球菌是最常见的细菌分离株。生物膜形成能力为这些物体上的生物体提供了额外的生存优势。目前的研究强调需要改善医护人员的手部卫生以及定期清洁/消毒经常与公众接触的场所。
Objects frequently touched by patients and healthcare workers in hospitals harbor potential pathogens and may act as source of infectious agents. This study aimed to determine the bacterial contamination of common hospital objects frequently touched by patients, visitors and healthcare workers. A total of 232 samples were collected from various sites like surface of biometric attendance devices, elevator buttons, door handles, staircase railings, telephone sets and water taps. Isolation, identification and antibiotic susceptibility testing of the isolates was performed by standard microbiological techniques. Biofilm forming ability of the S. aureus isolates was tested by a microtitre plate method. A total of 232 samples were collected and 219 bacterial isolates were recovered from 181 samples. Staphylococcus aureus was the most common bacterial isolate (44/219). Majority of S. aureus isolates were recovered from elevator buttons, biometric attendance devices and door handles. Among the S. aureus isolates, 36.3% (16/44) were methicillin resistant Staphylococcus aureus (MRSA) while remaining were methicillin sensitive Staphylococcus aureus (MSSA). Out of 44 S. aureus isolates, 12 (29.5%) were multidrug resistant and 14 (31.8%) were biofilm producers. The majority of MRSA isolates 62.5% (10/16) were biofilm producers. Acinetobacter was the most common Gram negative isolate followed by E coli and Pseudomonas species. High bacterial contamination of frequently touched objects with variety of potential pathogens and normal flora was detected. S. aureus was the most common bacterial isolate. Biofilm forming ability offers additional survival advantage to the organisms on these objects. Present study highlights the need of improved hand hygiene among healthcare workers and regular cleaning/disinfection of sites of frequent public contact.
登录
查看更多内容
影响因子：
5.5
作者：
Mann EE;Manna D;Mettetal MR;May RM;Dannemiller EM;Chung KK;Brennan AB;Reddy ST
通讯作者：
Reddy ST
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
影响因子：
2.8
作者：
Chaibenjawong, Plykaeow;Foster, Simon J.
通讯作者：
Foster, Simon J.
影响因子：
1.9
作者：
Uneke, Chigozie J.;Ogbonna, Annayo;Onu, Christian M.
通讯作者：
Onu, Christian M.
DOI：
10.1186/1476-0711-12-39
发表时间：
2013-12-13
影响因子：
5.7
作者：
Shiferaw, Teklu;Beyene, Getenet;Sewunet, Tsegaye
通讯作者：
Sewunet, Tsegaye