A combined scoring method to assess behavioral recovery after mouse spinal cord injury.
A combined scoring method to assess behavioral recovery after mouse spinal cord injury.
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DOI：
10.1016/j.neures.2010.02.009
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发表时间：
2010-06
影响因子：
2.9
通讯作者：
Faden AI
中科院分区：
文献类型：
--
作者：
Pajoohesh-Ganji A;Byrnes KR;Fatemi G;Faden AI
虽然大鼠一直是研究实验性脊髓损伤(SCI)病理生理学和治疗的主要啮齿动物，但转基因动物的增加导致了更多的小鼠模型的使用。然而，与大鼠相比，小鼠脊髓损伤后的行为评估没有得到广泛的研究，也很少有研究严格地检查行为测试与损伤严重程度或组织损伤之间的相关性。本研究采用减重的方法研究了T9脊髓挫伤后C57BL/6小鼠后肢功能的变化。关于可变性、评分者间的可靠性以及与损伤严重程度和脑白质保留的相关性，对许多行为测试进行了检查。将小鼠分为假损伤组、轻中度损伤组和中重度损伤组，分别于损伤后第1天和每周进行Basso小鼠评分(BMS)、爬梯实验、网格行走实验、斜面实验、足底实验和甩尾实验。阶梯攀登和栅格行走测试被证明不适合在小鼠身上使用，但修改后增强了它们在损伤严重程度方面的预测价值。斜面试验、足底试验和甩尾试验显示了太多的变异性，没有有意义的预测价值。正如之前报道的那样，BMS评分被证明是可靠的，但使用BMS、梯形爬坡(改良)和握力行走(改良格栅行走)的综合评分(BLG)提供了更好的损伤程度分离，并且比单独测试的变异性更小。这些数据为使用联合评分方法跟踪小鼠脊髓挫伤后的运动恢复提供了支持。
Although the rat has been the predominant rodent used to investigate the pathophysiology and treatment of experimental spinal cord injury (SCI), the increasing availability of transgenic animals has led to greater use of mouse models. However, behavioral assessment after SCI in mice has been less extensively investigated than in rats and few studies have critically examined the correlation between behavioral tests and injury severity or tissue damage. The present study characterized hind-limb functional performance in C57Bl/6 mice after contusion SCI at T9 using the weight drop method. A number of behavioral tests were examined with regard to variability, inter-rater reliability, and correlation to injury severity and white matter sparing. Mice were subjected to sham, mild-moderate or moderate-severe SCI and evaluated at day 1 and weekly up to 42 days using the Basso mouse scale (BMS), ladder climb, grid walk, inclined plane, plantar test and tail flick tests. The ladder climb and grid walk tests proved sub-optimal for use in mice, but modifications enhanced their predictive value with regard to injury severity. The inclined plane, plantar test and tail flick test showed far too much variability to have meaningful predictive value. The BMS score proved reliable, as previously reported, but a combined score (BLG) using BMS, Ladder climb (modified), and Grip walk (modified grid walk) provided better separation across injury levels and less variability than the individual tests. These data provide support for use of a combined scoring method to follow motor recovery in mice after SCI contusion injury.
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