Antibacterial and anticancer PDMS surface for mammalian cell growth using the Chinese herb extract paeonol(4-methoxy-2-hydroxyacetophenone).
Antibacterial and anticancer PDMS surface for mammalian cell growth using the Chinese herb extract paeonol(4-methoxy-2-hydroxyacetophenone).
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DOI：
10.1038/srep38973
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发表时间：
2016-12-12
影响因子：
4.6
通讯作者：
Feng X
中科院分区：
文献类型：
--
作者：
Jiao J;Sun L;Guo Z;Hou S;Holyst R;Lu Y;Feng X
聚二甲基硅氧烷(PDMS)被广泛用作细胞培养平台，用于制造基于微纳技术的微器件。然而，天然的PDMS表面不适合细胞黏附，容易受到细菌污染和癌细胞侵袭。在PDMS表面涂覆抗菌或抗癌材料往往会对其上的非癌症哺乳动物细胞造成相当大的伤害。我们开发了一种制备生物相容性PDMS表面的方法，通过在PDMS表面涂覆一种中草药提取物丹皮酚，不仅促进非癌症哺乳动物细胞的生长，而且具有抗菌和抗癌活性。涂层改变了PDMS表面的润湿性和元素组成。分子动力学模拟表明丹皮酚在PDMS表面的吸附是一个能量有利的过程。丹皮酚包覆的PDMS表面对革兰氏阳性菌和革兰氏阴性菌均表现出良好的抗菌活性。此外，涂层表面在漂洗或在水中孵化后仍保持相当大的抗菌活性。涂覆的PDMS表面抑制了接触表面的细菌生长，促进了非癌症哺乳动物细胞的生长，细胞毒性低，同时显着抑制了癌细胞的生长。我们的研究将有可能指导PDMS表面改性方法来生产生物医学设备。
Polydimethylsiloxane (PDMS) is widely used as a cell culture platform to produce micro- and nano-technology based microdevices. However, the native PDMS surface is not suitable for cell adhesion and is always subject to bacterial pollution and cancer cell invasion. Coating the PDMS surface with antibacterial or anticancer materials often causes considerable harm to the non-cancer mammalian cells on it. We have developed a method to fabricate a biocompatible PDMS surface which not only promotes non-cancer mammalian cell growth but also has antibacterial and anticancer activities, by coating the PDMS surface with a Chinese herb extract, paeonol. Coating changes the wettability and the elemental composition of the PDMS surface. Molecular dynamic simulation indicates that the absorption of paeonol onto the PDMS surface is an energy favourable process. The paeonol-coated PDMS surface exhibits good antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover considerable antibacterial activity is maintained after the coated surface is rinsed or incubated in water. The coated PDMS surface inhibits bacterial growth on the contact surface and promotes non-cancer mammalian cell growth with low cell toxicity; meanwhile the growth of cancer cells is significantly inhibited. Our study will potentially guide PDMS surface modification approaches to produce biomedical devices.
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影响因子：
16.6
作者：
Jakiela, Slawomir;Kaminski, Tomasz S.;Cybulski, Olgierd;Weibel, Douglas B.;Garstecki, Piotr
通讯作者：
Garstecki, Piotr
影响因子：
3.7
作者：
Ou Y;Li Q;Wang J;Li K;Zhou S
通讯作者：
Zhou S
用于受控细胞培养的 L-3,4-二羟基苯丙氨酸胶原蛋白修饰的 PDMS 表面
影响因子：
--
作者：
Gao, Chun-Ying;Guo, Yuan-Yuan;Feng, Xi-Zeng
通讯作者：
Feng, Xi-Zeng
中药提取物对聚二甲基硅氧烷表面的杀菌：绿原酸的抗菌新机制
影响因子：
4.6
作者：
Ren S;Wu M;Guo J;Zhang W;Liu X;Sun L;Holyst R;Hou S;Fang Y;Feng X
通讯作者：
Feng X
影响因子：
4.4
作者：
FELLER, SE;ZHANG, YH;BROOKS, BR
通讯作者：
BROOKS, BR