Antitumor and Apoptosis Induction Effects of Paeonol on Mice Bearing EMT6 Breast Carcinoma.
Antitumor and Apoptosis Induction Effects of Paeonol on Mice Bearing EMT6 Breast Carcinoma.
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DOI：
10.4062/biomolther.2013.106
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发表时间：
2014-07
影响因子：
3.7
通讯作者：
Zhou S
中科院分区：
文献类型：
--
作者：
Ou Y;Li Q;Wang J;Li K;Zhou S
丹皮酚是中药黄柏的主要酚类小分子成分。它在以前的研究中显示出抗肿瘤作用;然而，其潜在机制仍然未知。本研究通过给予安慰剂、环磷酰胺、丹皮酚150和300 mg/kg治疗4组荷EMT 6乳腺癌小鼠来探讨其机制。形态学分析，包括苏木精-伊红染色和TUNEL染色证实肿瘤细胞凋亡。结果显示，在150和300 mg/kg的丹皮酚处理组中，EMT 6乳腺肿瘤的重量显著降低。免疫组化和Western blot结果显示，Bcl-2表达下调，Bax、caspase 8和caspase 3表达上调。这些结果表明，丹皮酚具有抗肿瘤作用，其抑制机制是通过诱导细胞凋亡，调节Bcl-2和Bax的表达，激活caspase 8和caspase 3。
Paeonol is a major phenolic micromolecular component of Moutan cortex Radicis, a traditional Chinese Medicine. It has shown antitumor effects in previous studies; however, the underlying mechanisms remain unknown. This study investigated the mechanism by giving treatments of placebo, cyclophosphamide, paeonol of 150 and 300 mg/kg to 4 groups of mice bearing EMT6 breast cancer. Apoptosis in tumor cells were confirmed by morphology analysis, including hematoxylin, eosin staining and TUNEL staining. The results showed that the weight of EMT6 breast tumor was significantly reduced in the groups treated with both 150 and 300 mg/kg of paeonol. Immunohistochemical and Western blot results showed that the expression of Bcl-2 was down-regulated while the expression of Bax, caspase 8 and caspase 3 was up-regulated respectively. These results suggest that paeonol exhibits antitumor effects and the mechanism of the inhibition is via induction of apoptosis, regulation of Bcl-2 and Bax expression, and activation of caspase 8 and caspase 3.
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影响因子：
1.6
作者：
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通讯作者：
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影响因子：
4.7
作者：
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影响因子：
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作者：
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作者：
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