Harvesting multipotent progenitor cells from a small sample of tonsillar biopsy for clinical applications.
Harvesting multipotent progenitor cells from a small sample of tonsillar biopsy for clinical applications.
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DOI：
10.1186/s13287-017-0619-x
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发表时间：
2017-07-27
影响因子：
7.5
通讯作者：
Zalzman M
中科院分区：
文献类型：
--
作者：
Khatri R;Arad M;Ortlip T;Portney BA;Meltzer WA;Diaconu S;Silipino LE;Wang Y;Kaetzel DM;Taylor RJ;Zalzman M
人类成体干细胞具有治愈多种疾病和退行性疾病的潜力。与多能干细胞相比，它们具有主要优势，因为它们可以来自任何年龄的供体，因此不会引起伦理问题或体内畸胎瘤肿瘤形成的风险。此外，它们具有分化和分泌促进组织愈合的因子的天然能力，而无需遗传操作。然而，目前，成体干细胞的临床应用受到缺乏可靠的、标准化的和容易获得的组织来源的限制，所述组织来源不依赖于从无关的外科手术中丢弃的标本。人扁桃体衍生的间充质祖细胞（MPC）从扁桃体组织的小样品（平均0.88cm 3）中分离。我们的新方法对组织造成最小的机械和酶损伤，因此导致高细胞活力和产量。我们表征了这些MPC，并在体外证明了强大的多能性。我们进一步表明，这些细胞可以在无异种条件下繁殖和维持。我们已经从不同年龄、性别和种族的多个供体中产生了扁桃体活检衍生的MPC（T-MPC）系，并成功地在培养中扩增了它们。我们通过细胞表面标记以及体外扩增和分化潜力对其进行了表征。我们的程序提供了一个强大的产量扁桃体活检衍生的T-MPC。可以从小于1 g的样品中收获数百万个MPC，该样品可以在门诊环境中从完全清醒的供体中收集，而无需全身麻醉或住院。我们的研究确定扁桃体活检是再生医学成人MPC的丰富来源。本文的在线版本（doi：10.1186/s13287-017-0619-x）包含补充材料，可供授权用户使用。
Human adult stem cells hold the potential for the cure of numerous conditions and degenerative diseases. They possess major advantages over pluripotent stem cells as they can be derived from donors at any age, and therefore pose no ethical concerns or risk of teratoma tumor formation in vivo. Furthermore, they have a natural ability to differentiate and secrete factors that promote tissue healing without genetic manipulation. However, at present, clinical applications of adult stem cells are limited by a shortage of a reliable, standardized, and easily accessible tissue source which does not rely on specimens discarded from unrelated surgical procedures. Human tonsil-derived mesenchymal progenitor cells (MPCs) were isolated from a small sample of tonsillar tissue (average 0.88 cm3). Our novel procedure poses a minimal mechanical and enzymatic insult to the tissue, and therefore leads to high cell viability and yield. We characterized these MPCs and demonstrated robust multipotency in vitro. We further show that these cells can be propagated and maintained in xeno-free conditions. We have generated tonsillar biopsy-derived MPC (T-MPC) lines from multiple donors across a spectrum of age, sex, and race, and successfully expanded them in culture. We characterized them by cell surface markers, as well as in vitro expansion and differentiation potential. Our procedure provides a robust yield of tonsillar biopsy-derived T-MPCs. Millions of MPCs can be harvested from a sample smaller than 1 g, which can be collected from a fully awake donor in an outpatient setting without the need for general anesthesia or hospitalization. Our study identifies tonsillar biopsy as an abundant source of adult MPCs for regenerative medicine. The online version of this article (doi:10.1186/s13287-017-0619-x) contains supplementary material, which is available to authorized users.
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影响因子：
3.3
作者：
Ding, Dah-Ching;Shyu, Woei-Cherng;Lin, Shinn-Zong
通讯作者：
Lin, Shinn-Zong
影响因子：
3.6
作者：
Gargett, Caroline E.;Schwab, Kjiana E.;Wu, Di
通讯作者：
Wu, Di
影响因子：
4.9
作者：
Janjanin S;Djouad F;Shanti RM;Baksh D;Gollapudi K;Prgomet D;Rackwitz L;Joshi AS;Tuan RS
通讯作者：
Tuan RS
影响因子：
5.6
作者：
Jin, Hye Jin;Bae, Yun Kyung;Chang, Jong Wook
通讯作者：
Chang, Jong Wook
DOI：
10.1111/j.1365-2184.1970.tb00347.x
发表时间：
1970-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
通讯作者：
LALYKINA, KS