Escherichia coli killing by epidemiologically successful sublineages of Shigella sonnei is mediated by colicins.
Escherichia coli killing by epidemiologically successful sublineages of Shigella sonnei is mediated by colicins.
复制标题
DOI：
10.1016/j.ebiom.2023.104822
复制
发表时间：
2023-11
期刊：
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
志贺氏菌属是肠道病原体，每年导致超过 1.25 亿例志贺氏菌病。宋内链球菌约占这些病例的四分之一，并且在工业化国家中越来越流行。作为一种肠道病原体，宋内链球菌受益于战胜大肠杆菌等肠道共生菌，从而建立自身并引起疾病。细菌病原体利用多种机制来战胜竞争对手，其中包括称为大肠菌素的分子。最近，6 型分泌系统 (T6SS) 被描述为有助于杀死宋内链球菌中的大肠杆菌。我们使用流行病学重复的批量表型分析 (BPER)，将细菌全基因组关联研究 (bGWAS) 和高通量表型分析结合起来，对一系列宋内链球菌监测分离株进行分析，以确定与杀死大肠杆菌相关的遗传特征，并探索它们与流行病学行为的关系。我们利用基因组学、实验室实验和蛋白质组学进一步探讨了分离株中大肠杆菌素和 T6SS 成分的存在。我们的 bGWAS 分析返回了与大肠杆菌杀灭显着相关的已知和新型大肠菌素以及大肠菌素相关基因。计算机分析确定了与流行病学成功的亚谱系相关的杀伤表型的关键大肠杆菌素簇。杀伤表型与 T6SS 的存在无关。实验室分析证实了关键大肠杆菌素簇的存在，并且杀灭作用与接触无关。大肠杆菌素负责被宋内链球菌杀死，而不是 T6SS。这种表型有助于形成所观察到的宋内链球菌流行病学，并可能导致其在全球范围内的流行率不断上升。 BPER 是一种流行病学相关的表型测试方法，能够快速识别表型变化的遗传驱动因素，并评估其与自然环境中流行病学的相关性。 ， ， ， （英国）， 。
Shigella sp. are enteric pathogens which causes >125 million cases of shigellosis annually. S. sonnei accounts for about a quarter of those cases and is increasingly prevalent in industrialising nations. Being an enteric pathogen, S. sonnei benefits from outcompeting gut commensals such as Escherichia coli to establish itself and cause disease. There are numerous mechanisms that bacterial pathogens use to outcompete its rivals including molecules called colicins. A Type 6 Secretion System (T6SS) was recently described as contributing to E. coli killing in S. sonnei. We used Bulk Phenotyping of Epidemiological Replicates (BPER) which combined bacterial Genome Wide Association Studies (bGWAS) and high throughput phenotyping on a collection of S. sonnei surveillance isolates to identify the genetic features associated with E. coli killing and explore their relationship with epidemiological behaviour. We further explored the presence of colicins and T6SS components in the isolates using genomics, laboratory experimentation, and proteomics. Our bGWAS analysis returned known and novel colicin and colicin related genes as significantly associated with E. coli killing. In silico analyses identified key colicin clusters responsible for the killing phenotype associated with epidemiologically successful sub-lineages. The killing phenotype was not associated with the presence of a T6SS. Laboratory analyses confirmed the presence of the key colicin clusters and that killing was contact-independent. Colicins are responsible for E. coli killing by S. sonnei, not a T6SS. This phenotype contributes to shaping the observed epidemiology of S. sonnei and may contribute to its increasing prevalence globally. BPER is an epidemiologically relevant approach to phenotypic testing that enables the rapid identification of genetic drivers of phenotypic changes, and assessment of their relevance to epidemiology in natural settings. , , , (UK), .
登录
查看更多内容
影响因子：
16.6
作者：
Hawkey J;Paranagama K;Baker KS;Bengtsson RJ;Weill FX;Thomson NR;Baker S;Cerdeira L;Iqbal Z;Hunt M;Ingle DJ;Dallman TJ;Jenkins C;Williamson DA;Holt KE
通讯作者：
Holt KE
影响因子：
11.8
作者：
Lindsay B;Oundo J;Hossain MA;Antonio M;Tamboura B;Walker AW;Paulson JN;Parkhill J;Omore R;Faruque AS;Das SK;Ikumapayi UN;Adeyemi M;Sanogo D;Saha D;Sow S;Farag TH;Nasrin D;Li S;Panchalingam S;Levine MM;Kotloff K;Magder LS;Hungerford L;Sommerfelt H;Pop M;Nataro JP;Stine OC
通讯作者：
Stine OC
影响因子：
11.8
作者：
Ingle, Danielle J.;Easton, Marion;Williamson, Deborah A.
通讯作者：
Williamson, Deborah A.
Shigella多样性和不断变化的景观：二十一世纪的见解。
影响因子：
5.7
作者：
Anderson M;Sansonetti PJ;Marteyn BS
通讯作者：
Marteyn BS
影响因子：
12.3
作者：
Brynildsrud O;Bohlin J;Scheffer L;Eldholm V
通讯作者：
Eldholm V