Regeneration and Agrobacterium-mediated genetic transformation in Dianthus chinensis
Regeneration and Agrobacterium-mediated genetic transformation in Dianthus chinensis
复制标题
石竹再生及农杆菌介导的遗传转化
DOI：
10.1016/j.scienta.2021.110279
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发表时间：
2021-09
影响因子：
4.3
通讯作者：
Fu Xiaopeng
中科院分区：
文献类型：
--
作者：
Zhang Xiaoni;Wu Quanshu;Lin Shengnan;Zhang Zhen;Wang Zehao;Wang Qijian;Yan Xiuli;Bendahmane Mohammed;Bao Manzhu;Fu Xiaopeng
石竹是一种广泛应用于岩石园林的观赏植物，具有很高的商业价值。然而，关于该植物的再生和遗传转化的研究报道很少。在本研究中，D.以中华小球藻为外植体进行再生和农杆菌侵染。结果表明，1-萘乙酸（NAA）能显著提高再生效率。将子叶与含有pK 7 GWIWG 2-GFP质粒的根癌农杆菌EHA 105共培养15-20分钟。经卡那霉素（80 mg·L-1）筛选获得转基因再生植株。qRT-PCR结果证实了GFP和与细胞分裂相关的DcIPT 1基因在转DcIPT 1植株中的表达。中国。结果表明，转DcIPT 1基因植株的增殖能力增强。总之，本研究成功建立了一种可靠、高效的基于农杆菌侵染和植株再生相结合的中国石斛子叶转化和植株再生方法。本研究为石竹属植物的功能基因研究和遗传改良提供了理论依据。
Dianthus chinensisis widely used as a landscaping plant in rock gardens and has high commercial value. However, few studies of this plant's regeneration and genetic transformation have been reported. In this study, cotyledons ofD. chinensiswere used as explants for regeneration and infection withAgrobacterium. The results indicated that the efficiency of regeneration was markedly improved by using 1-naphthylcetic acid (NAA). The cotyledons were cocultured withAgrobacterium tumefaciensEHA105 containing pK7GWIWG2-GFP plasmids for 15–20 min. Transgenic regenerated plants were obtained by kanamycin (80 mg·L−1) screening. qRT-PCR results confirmed the expression ofGFPand theDcIPT1(which is related to cell division) gene in the transgenicDcIPT1plants ofD. chinensis. The results show that the proliferation ability of transgenicDcIPT1plants is enhanced. Overall, we report the successful establishment of a reliable and efficient method for cotyledon transformation and plant regeneration inD. chinensisbased on the combination of infection withAgrobacteriumand plant regeneration. This work will contribute to functional gene research and genetic improvement ofDianthusspecies plants.
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DOI：
--
发表时间：
2011
期刊：
Advances in Applied Science Research
影响因子：
--
作者：
J. K. Kanwar;Surinder Kumar
通讯作者：
J. K. Kanwar;Surinder Kumar
影响因子：
4.3
作者：
Abbasi, Hojatollah;Naderi, Roohangiz;Okazaki, Keichii
通讯作者：
Okazaki, Keichii
DOI：
10.1007/bf00048762
发表时间：
1996-05
期刊：
Plant Cell, Tissue and Organ Culture
影响因子：
--
作者：
A. V. Altvorst;H. Koehorst;J. Jong;H. Dons
通讯作者：
A. V. Altvorst;H. Koehorst;J. Jong;H. Dons
影响因子：
4.3
作者：
Xiaopeng Fu;Jingjing Zhang;Feng Li;PinTing Zhan;M. Bao
通讯作者：
Xiaopeng Fu;Jingjing Zhang;Feng Li;PinTing Zhan;M. Bao
DOI：
10.1080/14620316.2002.11511532
发表时间：
2002-01
期刊：
The Journal of Horticultural Science and Biotechnology
影响因子：
--
作者：
C. Nontaswatsri;S. Fukai;T. Touma;M. Goi
通讯作者：
C. Nontaswatsri;S. Fukai;T. Touma;M. Goi