Three dimensional dental epithelial-mesenchymal constructs of predetermined size and shape for tooth regeneration.
Three dimensional dental epithelial-mesenchymal constructs of predetermined size and shape for tooth regeneration.
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DOI：
10.1016/j.biomaterials.2010.07.020
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Yelick, Pamela C.
中科院分区：
文献类型：
--
作者：
Zhang, Weibo;Ahluwalia, Ivy P.;Yelick, Pamela C.
关键词：
虽然已知精确的牙上皮-间充质(DE-DM)细胞相互作用在牙齿发育中起关键作用，但建立适当的DE-DM细胞相互作用以促进牙齿再生的可靠方法尚未建立。为了解决这一挑战，并产生预定大小和形状的生物工程牙齿，在这项研究中，我们表征了三维(3D)预制DE-DM细胞结构。将人牙髓细胞种植的胶原凝胶层与猪DE细胞悬浮在生长因子降低(GFR)Matrigel中共培养。将所得到的3D DE-DM细胞层进行体外培养，或植入裸鼠体内皮下生长。分子、组织学和免疫组织化学(IHC)分析显示，4周后DE-DM细胞有组织的相互作用，牙组织特异性标记物釉原蛋白(AM)和牙本质涎磷蛋白(DSPP)以及基底膜标记层粘连蛋白5和IV型胶原被诱导表达，并有不规则的矿化组织形成。我们预计，这些研究将有助于最终建立可靠的方法来精心制作牙齿组织，以及特定大小和形状的全尺寸牙齿。
While it is known that precise dental epithelial-mesenchymal (DE-DM) cell interactions provide critical functions in tooth development, reliable methods to establish proper DE-DM cell interactions for tooth regeneration have yet to be established. To address this challenge, and to generate bioengineered teeth of predetermined size and shape, in this study, we characterize three-dimensional (3D) pre-fabricated DE-DM cell constructs. Human dental pulp cell seeded Collagen gel layers were co-cultured with porcine DE cells suspended in Growth Factor Reduced (GFR) Matrigel. The resulting 3D DE-DM cell layers were cultured in vitro, or implanted and grown subcutaneously in vivo in nude rats. Molecular, histological and immunohistochemical (IHC) analyses of harvested implants revealed organized DE-DM cell interactions, the induced expression of dental tissue-specific markers Amelogenin (AM) and Dentin Sialophosphoprotein (DSPP), and basement membrane markers Laminin 5 and collagen IV, and irregular mineralized tissue formation after 4 weeks. We anticipate that these studies will facilitate the eventual establishment of reliable methods to elaborate dental tissues, and full sized teeth of specified sized and shape.
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影响因子：
3
作者：
KUO, MYP;LAN, WH;HAHN, LJ
通讯作者：
HAHN, LJ
影响因子：
48
作者：
Nakao, Kazuhisa;Morita, Ritsuko;Tsuji, Takashi
通讯作者：
Tsuji, Takashi
DOI：
10.1007/978-1-60761-380-0_24
发表时间：
2010-01-01
期刊：
EPIDERMAL CELLS: METHODS AND PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Egles, Christophe;Garlick, Jonathan A.;Shamis, Yulia
通讯作者：
Shamis, Yulia
影响因子：
3.7
作者：
Laoide, BM;Gastinne, I;Rougeon, F
通讯作者：
Rougeon, F
DOI：
10.1073/pnas.0902944106
发表时间：
2009-08-11
影响因子：
11.1
作者：
Ikeda, Etsuko;Morita, Ritsuko;Tsuji, Takashi
通讯作者：
Tsuji, Takashi