Plasma Amyloid Beta Concentrations in Aged and Cognitively Impaired Pet Dogs.
Plasma Amyloid Beta Concentrations in Aged and Cognitively Impaired Pet Dogs.
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老年和认知障碍宠物狗的血浆β淀粉样蛋白浓度。
DOI：
10.1007/s12035-020-02140-9
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发表时间：
2021-03
影响因子：
5.1
通讯作者：
Olby NJ
中科院分区：
文献类型：
--
作者：
Panek WK;Murdoch DM;Gruen ME;Mowat FM;Marek RD;Olby NJ
长寿相关的神经系统疾病已经在人类和犬类老年人群中被观察到。阿尔茨海默病（AD）和犬认知功能障碍综合征（CDS）代表了随着年龄增长而影响这两个物种的可比疾病。需要对这些不治之症进行转化诊断和治疗研究。淀粉样蛋白β (Aβ)肽家族是与ad相关的肽，在狗和人之间具有相同的氨基酸序列。人血浆Aβ42浓度随年龄增加而降低，脑脊液（CSF）浓度随AD降低，且与脑内淀粉样蛋白负荷呈负相关。同样，患有CDS的狗的脑脊液Aβ42浓度下降，但关于衰老狗和患有CDS的狗的血浆Aβ42浓度的信息有限且相互矛盾。我们用超灵敏单分子阵列法（SIMOA）测定了不同生命阶段的健康衰老犬（n=36）和患有CDS的犬（n=11）的血浆中a β42和a β40的浓度。并计算了Aβ42/β40的比值。a - β42和a - β40的平均血浆浓度随年龄的增长而升高（r2 =0.27, p=0.001和r2 =0.42, p<0.001），随年龄的增长而升高：幼犬/少年组（0.43-2岁）分别为1.23±0.95和38.26±49.43pg/mL；成人/成熟组（2.1 ~ 9岁）：10.99±5.45和131.05±80.17pg/mL；老年组（9.3 ~ 14.5岁）：分别为18.65±16.65和192.88±146.38pg/mL。CDS犬（11.0 ~ 15.6岁）Aβ42和Aβ40浓度分别为11.61±6.39和150.23±98.2pg/mL，显著低于同龄健康犬（p=0.0048和p=0.001）。我们的研究结果表明，犬血浆淀粉样蛋白浓度的动态与老年人类（有或没有AD）的动态相似。
Longevity associated neurological disorders have been observed across human and canine aging populations. Alzheimer’s disease (AD) and Canine Cognitive Dysfunction Syndrome (CDS) represent comparable diseases affecting both species as they age. Translational diagnostic and therapeutic research is needed for these incurable diseases. The amyloid β (Aβ) peptide family are AD-associated peptides with identical amino acid sequence between dogs and humans. Plasma Aβ42 concentration increases with age and decreases with AD in humans, and cerebrospinal fluid (CSF) concentration decreases in AD and correlates inversely with the amyloid load within the brain. Similarly, CSF Aβ42 concentrations decrease in dogs with CDS but there is limited and conflicting information on plasma Aβ42 concentrations in aging dogs and dogs with CDS. We measured plasma concentrations of Aβ42 and Aβ40 with an ultrasensitive single-molecule array assay (SIMOA) in a population of healthy aging dogs of different life-stages (n=36) and dogs affected with CDS (n=11). In addition, the ratio of Aβ42/β40 was calculated. The mean plasma concentrations of Aβ42 and Aβ40 increased significantly with age (r2 =0.27, p=0.001 and r2 =0.42, p<0.001 respectively) and with life stage: puppy/junior group, (0.43–2 years): 1.23±0.95 and 38.26±49.43pg/mL; adult/mature group, (2.1– 9 years):10.99±5.45 and 131.05±80.17pg/mL; geriatric/senior group, (9.3–14.5 years):18.65±16.65 and 192.88±146.38pg/mL, respectively. Concentrations of Aβ42 and Aβ40 in dogs with CDS (11.0–15.6 years) were significantly lower than age matched healthy dogs at 11.61±6.39 and 150.23±98.2pg/mL, (p=0.0048 and p=0.001) respectively. Our findings suggest the dynamics of canine plasma amyloid concentrations are analogous to that found in aging humans with and without AD.
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影响因子：
4.2
作者：
Colle, MA;Hauw, JJ;Duykaerts, C
通讯作者：
Duykaerts, C
影响因子：
2.9
作者：
Rofina, JE;van Ederen, AM;Gruys, E
通讯作者：
Gruys, E
影响因子：
3.5
作者：
Chow, Vivian W.;Mattson, Mark P.;Wong, Philip C.;Gleichmann, Marc
通讯作者：
Gleichmann, Marc
影响因子：
2.7
作者：
Cummings, BJ;Head, E;Cotman, CW
通讯作者：
Cotman, CW
DOI：
10.1016/j.bbadis.2013.03.016
发表时间：
2013-09
影响因子：
6.2
作者：
Head, Elizabeth
通讯作者：
Head, Elizabeth