Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the Southern Hemisphere.
Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the Southern Hemisphere.
复制标题
DOI：
10.1111/1462-2920.12310
复制
发表时间：
2014-04
影响因子：
5.1
通讯作者：
Cabello FC
中科院分区：
文献类型：
--
作者：
Ivanova LB;Tomova A;González-Acuña D;Murúa R;Moreno CX;Hernández C;Cabello J;Cabello C;Daniels TJ;Godfrey HP;Cabello FC
伯氏疏螺旋体（Borrelia burgdorferi）是莱姆病的病原，由蜱类传播。虽然蜱虫分布在北半球和南半球，但在除乌拉圭以外的南美洲缺乏伯氏疏螺旋体存在的证据。我们现在报告了在智利从环境植被和长尾稻鼠（Oligoryzomys longicaudatus）中收集的特有的stilesi蜱中存在具有平波形态和螺旋体DNA的可培养螺旋体。采用多位点序列分型和其他5个位点（16S和23S核糖体基因、5S-23S基因间间隔、flaB、ospC）测序对培养的蜱螺旋体和螺旋体DNA进行鉴定。系统发育分析将该螺旋体作为莱姆病组内的新基因种。经PCR和脉冲场凝胶电泳鉴定，其质粒谱与伯氏疏螺旋体B31A3不同。我们建议将这个莱姆博氏螺旋体的南美新成员命名为智利博氏螺旋体（Borrelia chilensis） VA1，以纪念其原产国。
Borrelia burgdorferi sensu lato (s.l.), transmitted by Ixodes spp. ticks, is the causative agent of Lyme disease. Although Ixodes spp. ticks are distributed in both Northern and Southern Hemispheres, evidence for the presence of B. burgdorferi s.l. in South America apart from Uruguay is lacking. We now report the presence of culturable spirochetes with flat-wave morphology and borrelial DNA in endemic Ixodes stilesi ticks collected in Chile from environmental vegetation and long-tailed rice rats (Oligoryzomys longicaudatus). Cultured spirochetes and borrelial DNA in ticks were characterized by multilocus sequence typing and by sequencing five other loci (16S and 23S ribosomal genes, 5S-23S intergenic spacer, flaB, ospC). Phylogenetic analysis placed this spirochete as a new genospecies within the Lyme borreliosis group. Its plasmid profile determined by PCR and pulsed-field gel electrophoresis differed from that of B. burgdorferi B31A3. We propose naming this new South American member of the Lyme borreliosis group Borrelia chilensis VA1, in honor of its country of origin.
登录
查看更多内容
影响因子：
2.1
作者：
Barbieri, Amalia M.;Venzal, Jose M.;Labruna, Marcelo B.
通讯作者：
Labruna, Marcelo B.
影响因子：
4.4
作者：
Benson, MJ;Gawronski, JD;Benson, DR
通讯作者：
Benson, DR
影响因子：
2.2
作者：
González-Acuña, D;Guglielmone, AA
通讯作者：
Guglielmone, AA
影响因子：
2.1
作者：
Balenghien, T.;Fouque, F.;Bicout, D. J.
通讯作者：
Bicout, D. J.
影响因子：
6.4
作者：
Barbour, Alan G.;Travinsky, Bridgit
通讯作者：
Travinsky, Bridgit