2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors.
2B4, a new member of the immunoglobulin gene superfamily, is expressed on murine dendritic epidermal T cells and plays a functional role in their killing of skin tumors.
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2B4是免疫球蛋白基因超家族的新成员，在鼠树突状表皮T细胞上表达，并在其杀死皮肤肿瘤的过程中发挥功能性作用。
DOI：
10.1111/1523-1747.ep12323533
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Takashima,A
中科院分区：
文献类型：
--
作者：
Schuhmachers,G;Ariizumi,K;Mathew,PA;Bennett,M;Kumar,V;Takashima,A
树突状表皮T细胞是组织驻留的γδT细胞家族中皮肤特异的成员，其特点是通过非主要组织相容性复合体限制性机制杀伤选定的肿瘤靶点。我们最近发现了一种新的受体分子2B4，它似乎与自然杀伤细胞对肿瘤靶点的非MHC限制性识别有关。这项研究的目的是确定DETC是否表达2B4分子，如果是的话，评估它们在DETC介导的肿瘤靶点杀伤中的功能作用。短期的DETC株系以及新鲜从皮肤获得的DETC表达表面2B4，用特定的单抗进行检测。从DETC培养物中去除白介素2可显著降低2B4的表达水平，并在标准的51Cr释放试验中降低对YAC-1靶点的细胞毒能力。相反，暴露于IL-2，而不是IL-7，会增加2B4的表达和细胞毒性。为了评估表面2B4的功能作用，我们用抗2B4抗体处理DETC细胞，然后检测它们的杀伤能力。抗2B4抗体，而不是对照抗体，增加了它们裂解YAC-1靶点的能力(51Cr释放试验)，并破坏了Pam-212转化的角质形成细胞的单层(视觉评估)。因此，我们得出结论，DETC以IL-2依赖的方式表达2B4分子，这可能在皮肤源性肿瘤的杀伤中发挥独特的作用。
Dendritic epidermal T cells (DETC), which are skin-specific members of the tissue-resident γδ T-cell family, are characterized by their potential to kill selected tumor targets by a non-major histocompatibility complex (MHC)-restricted mechanism. We have recently identified a new receptor molecule, 2B4, that appears to be associated with non-MHC-restricted recognition of tumor targets by natural killer cells. The purpose of this study was to determine whether DETC express 2B4 molecules, and, if so, to assess their functional roles in DETC-mediated killing of tumor targets. Short-term DETC lines as well as DETC freshly procured from skin expressed surface 2B4, as detected with a specific monoclonal antibody. Removal of interleukin (IL)-2 from DETC cultures caused substantial reduction in 2B4 expression levels as well as a reduction in cytotoxic capacity against YAC-1 targets in a standard51Cr-release assay. Conversely, exposure to IL-2, but not to IL-7, elevated both 2B4 expression and cytotoxicity. To assess the functional roles played by surface 2B4, we pretreated DETC lines with anti-2B4 antibody and then tested for their killing potential. Anti-2B4, but not the control antibody, augmented their capacity to lyse YAC-1 targets (51Cr-release assays) and to disrupt the monolayers of Pam-212-transformed keratinocytes (visual assessment). Thus, we conclude that DETC express, in an IL-2-dependent manner, 2B4 molecules, which may play a unique role in the killing of skin-derived tumors.
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DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nixon-Fulton,JL;Bergstresser,PR;Tigelaar,RE
通讯作者：
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DOI：
10.1111/1523-1747.ep12560835
发表时间：
1988
期刊：
The Journal of investigative dermatology
影响因子：
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通讯作者：
Tigelaar,RE
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