Thy-1+ dendritic epidermal cells in mice: precursor frequency analysis and cloning of concanavalin A-reactive cells.
Thy-1+ dendritic epidermal cells in mice: precursor frequency analysis and cloning of concanavalin A-reactive cells.
复制标题
小鼠 Thy-1 树突状表皮细胞：前体频率分析和伴刀豆球蛋白 A 反应细胞的克隆。
DOI：
10.1111/1523-1747.ep12560835
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Tigelaar,RE
中科院分区：
文献类型：
--
作者：
Takashima,A;Nixon-Fulton,JL;Bergstresser,PR;Tigelaar,RE
已证明小鼠Thy-1+树突状表皮细胞（Thy-1+DEC）的大量培养物会响应伴刀豆球蛋白A（Con A）和IL-2而增殖，分泌IL-2样生长因子，并溶解YAC- 1等靶细胞。利用有限稀释微量培养以确定AKR/J表皮细胞悬浮液中Con A-响应性Thy-1+DEC的前体频率以及这几种功能活性是否都存在于单个Thy-1+DEC前体中。用有限数量的FACS纯化的Thy-1+DEC、辐照的同源脾填充细胞和外源性IL-2建立的培养物的前体频率分析表明，约20%的Thy-1+DEC响应于Con A而增殖。将纯化的Thy-1+DEC以0.5个细胞/孔的密度铺板的平行微量培养物用于建立克隆。20个克隆进行了表征表型，其中10个也进行了测试，其能力增殖反应刀豆蛋白A或IL-2，分泌IL-2样生长因子，并表现出细胞毒性。所有克隆均为Thy-1+和L3 T4-，但尽管大多数也为Lyt-2-，但有几个克隆含有3%-18%的无光泽Lyt-2+细胞。功能研究表明，每个克隆显示所有上述功能活动，虽然与大量的定量变化。具有最高细胞毒性活性的克隆对Con A或IL-2的反应性相对较低，并且包括所有含有暗淡Lyt-2+细胞的克隆;相反，具有最高增殖反应的克隆具有相对较低的细胞毒性活性，并且均为Lyt-2-。克隆的Thy-1+DEC之间的这种程度的功能和表型异质性可能反映了它们特定的活化或分化状态;它是否反映了这些细胞的生物学相关的体内活性仍有待确定。
Bulk cultures of mouse Thy-1+dendritic epidermal cells (Thy-1+DEC) have been shown to proliferate in response to concanavalin A (Con A) and IL-2, to secrete IL-2-like growth factors, and to lyse target cells such as YAC- 1. Limiting dilution microculture was utilized in order to determine the precursor frequency of Con A-responsive Thy-1+DEC in suspensions of AKR/J epidermal cells as well as whether these several functional activities all reside within a single Thy-1+DEC precursor. Precursor frequency analysis of cultures established with limiting numbers of FACS-purified Thy-1+DEC, irradiated syngeneic splenic filler cells and exogenous IL-2 indicated that approximately 20% of Thy-1+DEC proliferated in response to Con A. Parallel microcultures in which purified Thy-1+DEC were plated at a density of 0.5 cells/well were used to establish clones. Twenty clones were characterized phenotypically, and ten of these were also tested for their capacities to proliferate in response to Con A or IL-2, to secrete IL-2-like growth factors, and to exhibit cytotoxicity. All clones were Thy-1+and L3T4-, but while most were also Lyt-2-, several contained 3%-18% dull Lyt-2+cells. Functional studies revealed that each clone displayed all of the above functional activities, albeit with substantial quantitative variation. Clones with the highest cytotoxic activity had relatively low responsiveness to Con A or IL-2 and included all clones containing dull Lyt-2+cells; conversely, clones with the highest proliferative responses had relatively low cytotoxic activity and were all Lyt-2-. This degree of functional and phenotypic heterogeneity among cloned Thy-1+DEC may reflect their particular states of activation or differentiation; whether it reflects the biologically relevant in vivo activities of these cells must still be determined.
登录
查看更多内容
Thy-1 表皮细胞响应刀豆球蛋白 A 和白细胞介素 2 而增殖。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nixon-Fulton,JL;Bergstresser,PR;Tigelaar,RE
通讯作者：
Tigelaar,RE
对刀豆球蛋白 A 诱导的 TCGF 反应性 T 细胞的定量研究。
影响因子：
4.4
作者：
E. Larsson;K. Lindahl;Jean Langhorne;Antonio Coutinho
通讯作者：
Antonio Coutinho
检测异常 Thy-1 决定簇的单克隆抗体。
影响因子：
4.4
作者：
H. Auchincloss;K. Ozato;D. Sachs
通讯作者：
D. Sachs
影响因子：
4.4
作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
通讯作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
影响因子：
64.8
作者：
LANIER, LL;WEISS, A
通讯作者：
WEISS, A