Field-portable pixel super-resolution colour microscope.
Field-portable pixel super-resolution colour microscope.
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DOI：
10.1371/journal.pone.0076475
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Greenbaum A;Akbari N;Feizi A;Luo W;Ozcan A
基于部分相干数字直线全息，我们报道了一种野外便携式显微镜，可以在例如bbb20 mm2的宽视场上呈现无透镜彩色图像。这款计算全息显微镜重量不到145克，尺寸小于17×6×5厘米，特别适合现场设置和护理点使用。在这种无透镜成像设计中，我们将着色算法与基于源移位的多高度像素超分辨率技术相结合，以减轻全息成像中典型的“彩虹”样彩色伪影。该图像处理方案基于将RGB图像的颜色分量转换为YUV颜色空间，将图像的颜色信息与亮度分量分离。我们的超分辨率彩色显微镜的分辨率使用USAF测试图进行表征，以确认亚微米空间分辨率，甚至用于采用多高度相位恢复来处理密集和连接物体的重建。为了进一步证明该彩色显微镜的性能，巴氏涂片也成功成像。这种野外便携式和宽视场计算彩色显微镜可用于资源贫乏环境中的远程医疗应用。
Based on partially-coherent digital in-line holography, we report a field-portable microscope that can render lensfree colour images over a wide field-of-view of e.g., >20 mm2. This computational holographic microscope weighs less than 145 grams with dimensions smaller than 17×6×5 cm, making it especially suitable for field settings and point-of-care use. In this lensfree imaging design, we merged a colorization algorithm with a source shifting based multi-height pixel super-resolution technique to mitigate ‘rainbow’ like colour artefacts that are typical in holographic imaging. This image processing scheme is based on transforming the colour components of an RGB image into YUV colour space, which separates colour information from brightness component of an image. The resolution of our super-resolution colour microscope was characterized using a USAF test chart to confirm sub-micron spatial resolution, even for reconstructions that employ multi-height phase recovery to handle dense and connected objects. To further demonstrate the performance of this colour microscope Papanicolaou (Pap) smears were also successfully imaged. This field-portable and wide-field computational colour microscope could be useful for tele-medicine applications in resource poor settings.
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影响因子：
6.1
作者：
Bishara W;Sikora U;Mudanyali O;Su TW;Yaglidere O;Luckhart S;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Biener G;Greenbaum A;Isikman SO;Lee K;Tseng D;Ozcan A
通讯作者：
Ozcan A
影响因子：
3.8
作者：
Greenbaum A;Ozcan A
通讯作者：
Ozcan A
影响因子：
6.1
作者：
Balsam, Joshua;Ossandon, Miguel;Rasooly, Avraham
通讯作者：
Rasooly, Avraham
影响因子：
6.1
作者：
Gallegos, Dustin;Long, Kenneth D.;Cunningham, Brian T.
通讯作者：
Cunningham, Brian T.