Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level.
Human myogenic endothelial cells exhibit chondrogenic and osteogenic potentials at the clonal level.
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DOI：
10.1002/jor.22335
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发表时间：
2013-07
影响因子：
2.8
通讯作者：
Huard, Johnny
中科院分区：
文献类型：
--
作者：
Zheng, Bo;Li, Guangheng;Chen, William C. W.;Deasy, Bridget M.;Pollett, Jonathan B.;Sun, Bin;Drowley, Lauren;Gharaibeh, Burhan;Usas, Arvydas;Peault, Bruno;Huard, Johnny
我们之前已经报道了小鼠肌肉来源的干细胞(MMDSCs)具有很高的再生潜力，能够分化为多个中胚层细胞系，包括肌源性细胞、内皮细胞、软骨细胞和成骨细胞。最近，我们描述了一种可能的人类mMDSCs的对应物，即成人骨骼肌中的肌源性内皮细胞(MECs)，它能在动物疾病模型中有效地修复/再生受损和营养不良的骨骼肌以及缺血性心脏。然而，目前尚不清楚人骨髓间充质干细胞是否在克隆水平上保留了mMDSC样的软骨和成骨潜能，以及包括高增殖和抗应激在内的经典干细胞特性。在这里，我们证明了从新鲜的出生后人类骨骼肌活检组织中分离出来的微血管内皮细胞可以克隆生长，并对氧化应激表现出强大的抵抗力，而不会发生肿瘤。MEC克隆能够在体外诱导条件下分化为软骨细胞和成骨细胞，并参与体内软骨和骨的形成。此外，还观察了克隆性微血管内皮细胞(CMEC)的成脂和血管生成能力。综上所述，我们的研究表明，cMECs不仅表现出成体干细胞的典型特性，而且在体外和体内都具有成软骨和成骨能力，提示其在关节软骨和骨修复/再生方面具有潜在的应用前景。
We have previously reported the high regenerative potential of murine muscle-derived stem cells (mMDSCs) that are capable of differentiating into multiple mesodermal cell lineages, including myogenic, endothelial, chondrocytic, and osteoblastic cells. Recently, we described a putative human counterpart of mMDSCs, the myogenic endothelial cells (MECs), in adult human skeletal muscle, which efficiently repair/regenerate the injured and dystrophic skeletal muscle as well as the ischemic heart in animal disease models. Nevertheless it remained unclear whether human MECs, at the clonal level, preserve mMDSC-like chondrogenic and osteogenic potentials and classic stem cell characteristics including high proliferation and resistance to stress. Herein, we demonstrated that MECs, sorted from fresh postnatal human skeletal muscle biopsies, can be grown clonally and exhibit robust resistance to oxidative stress with no tumorigeneity. MEC clones were capable of differentiating into chondrocytes and osteoblasts under inductive conditions in vitro and participated in cartilage and bone formation in vivo. Additionally, adipogenic and angiogenic potentials of clonal MECs (cMECs) were observed. Overall, our study showed that cMECs not only display typical properties of adult stem cells but also exhibit chondrogenic and osteogenic capacities in vitro and in vivo, suggesting their potential applications in articular cartilage and bone repair/regeneration.
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DOI：
10.1083/jcb.200108150
发表时间：
2002-05-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Qu-Petersen Z;Deasy B;Jankowski R;Ikezawa M;Cummins J;Pruchnic R;Mytinger J;Cao B;Gates C;Wernig A;Huard J
通讯作者：
Huard J
DOI：
10.1083/jcb.150.5.1085
发表时间：
2000-09-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lee JY;Qu-Petersen Z;Cao B;Kimura S;Jankowski R;Cummins J;Usas A;Gates C;Robbins P;Wernig A;Huard J
通讯作者：
Huard J
影响因子：
46.9
作者：
Zheng, Bo;Cao, Baohong;Peault, Bruno
通讯作者：
Peault, Bruno
影响因子：
3.3
作者：
Drowley, Lauren;Okada, Masaho;Huard, Johnny
通讯作者：
Huard, Johnny
影响因子：
7.8
作者：
De Angelis, L;Berghella, L;Coletta, M;Lattanzi, L;Zanchi, M;Cusella-De Angelis, MG;Ponzetto, C;Cossu, G
通讯作者：
Cossu, G