Structural constraints in the packaging of bluetongue virus genomic segments.
Structural constraints in the packaging of bluetongue virus genomic segments.
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蓝肠病毒基因组段包装中的结构约束。
DOI：
10.1099/vir.0.066647-0
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Roy P
中科院分区：
文献类型：
--
作者：
Burkhardt C;Sung PY;Celma CC;Roy P
蓝舌病病毒（BTV）用于确保其10个基因组片段的分选和包装的机制仍然知之甚少。在这项研究中，我们调查了包装限制的两个BTV基因组片段从两个不同的血清型。将BTV血清9型的片段4（S4）依次突变，并通过两种新开发的RNA包装检测系统（一种在体内，另一种在体外）研究突变ssRNA的包装。对突变的ssRNA进行建模，然后进行生化数据分析，结果表明，分子5′和3′端相互作用形成的构象基序对于包装是必要的，也是足够的。在BTV血清1型的S8中也鉴定到类似的结构信号。此外，使用正义ssRNA的二级结构的相同构象分析来产生嵌合区段，其保持推定的包装基序，但含有不相关的内部序列。该嵌合片段被成功包装，证实所鉴定的基序指导片段的正确包装。
The mechanism used by bluetongue virus (BTV) to ensure the sorting and packaging of its 10 genomic segments is still poorly understood. In this study, we investigated the packaging constraints for two BTV genomic segments from two different serotypes. Segment 4 (S4) of BTV serotype 9 was mutated sequentially and packaging of mutant ssRNAs was investigated by two newly developed RNA packaging assay systems, one in vivo and the other in vitro. Modelling of the mutated ssRNA followed by biochemical data analysis suggested that a conformational motif formed by interaction of the 5′ and 3′ ends of the molecule was necessary and sufficient for packaging. A similar structural signal was also identified in S8 of BTV serotype 1. Furthermore, the same conformational analysis of secondary structures for positive-sense ssRNAs was used to generate a chimeric segment that maintained the putative packaging motif but contained unrelated internal sequences. This chimeric segment was packaged successfully, confirming that the motif identified directs the correct packaging of the segment.
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影响因子：
5.4
作者：
Boyce, Mark;Celma, Cristina C. P.;Roy, Polly
通讯作者：
Roy, Polly
影响因子：
5.4
作者：
SAMAL, SK;LIVINGSTON, CW;RAMIG, RF
通讯作者：
RAMIG, RF
DOI：
10.1073/pnas.1206069109
发表时间：
2012-06-05
影响因子：
11.1
作者：
Chou, Yi-ying;Vafabakhsh, Reza;Palese, Peter
通讯作者：
Palese, Peter
影响因子：
2.6
作者：
Roy, Polly
通讯作者：
Roy, Polly
影响因子：
3.8
作者：
RAMIG, RF;GARRISON, C;BELLROBINSON, D
通讯作者：
BELLROBINSON, D