The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways.
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways.
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DOI：
10.1038/s41467-017-02518-9
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发表时间：
2018-01-16
影响因子：
16.6
通讯作者：
Stams AJM
中科院分区：
文献类型：
--
作者：
Sousa DZ;Visser M;van Gelder AH;Boeren S;Pieterse MM;Pinkse MWH;Verhaert PDEM;Vogt C;Franke S;Kümmel S;Stams AJM
甲醇通常通过厌氧甲基化菌（如产甲烷菌和产醋菌）的含钴胺的甲醇甲基转移酶启动的途径代谢，或通过需氧菌使用甲醇脱氢酶氧化为甲醛。甲醇是深地下环境中的重要底物，其中Desulfotomaculum属的嗜热硫酸盐还原菌在其中起着关键作用。在这里，我们研究了从3000m深地热储层中分离的Desulfotomaculum kuznetsovii菌株17T的甲醇代谢。我们使用蛋白质组学来分析用甲醇和硫酸盐在存在和不存在钴和维生素B12的情况下生长的细胞。结果表明，D. kuznetsovii存在两种甲醇降解途径，即钴依赖的甲醇甲基转移酶和钴不依赖的甲醇脱氢酶，并通过稳定同位素分离进一步证实了这一点。这是利用两种不同的甲醇转化途径的微生物的第一份报告。我们假设这使库兹涅佐夫号在其自然环境中具有竞争优势。微生物利用甲醇甲基转移酶或甲醇脱氢酶代谢甲醇。在这里，作者使用蛋白质组学和稳定同位素分馏法表明，从地下深处分离出来的嗜热硫酸盐还原细菌使用这两种途径。
Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17T, isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment. Microorganisms metabolise methanol using either a methanol methyltransferase or a methanol dehydrogenase. Here, the authors use proteomics and stable isotope fractionation to show that a thermophilic sulfate-reducing bacterium, isolated from the deep subsurface, uses both pathways.
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影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
2.8
作者：
KLEMPS, R;CYPIONKA, H;PFENNIG, N
通讯作者：
PFENNIG, N
影响因子：
5
作者：
Balk, Melike;Weijma, Jan;Stams, Alfons J. M.
通讯作者：
Stams, Alfons J. M.
影响因子：
7.4
作者：
Gilevska, Tetyana;Gehre, Matthias;Richnow, Hans Hermann
通讯作者：
Richnow, Hans Hermann
DOI：
10.1007/10_2013_257
发表时间：
2014-01-01
期刊：
GEOBIOTECHNOLOGY II: ENERGY RESOURCES, SUBSURFACE TECHNOLOGIES, ORGANIC POLLUTANTS AND MINING LEGAL PRINCIPLES
影响因子：
--
作者：
Gniese, Claudia;Bombach, Petra;Krueger, Martin
通讯作者：
Krueger, Martin