Method development for fecal lipidomics profiling.
Method development for fecal lipidomics profiling.
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DOI：
10.1021/ac303011k
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发表时间：
2013-01-15
影响因子：
7.4
通讯作者：
Kristal BS
中科院分区：
文献类型：
--
作者：
Gregory KE;Bird SS;Gross VS;Marur VR;Lazarev AV;Walker WA;Kristal BS
用于分析粪便物质的强大方法将有助于了解肠道（病理）生理学及其在健康和疾病中的作用，并有助于改善对个体患者的护理，特别是早产儿等高危人群。由于脂质组学提供了一种生物学和分析上有吸引力的方法，我们开发了一种简单，灵敏，定量精确的方法来分析粪便中的完整脂质。该方法使用两种单独的互补萃取化学物质，二氯甲烷（DCM）和甲基叔丁基醚/六氟异丙醇（MTBE）混合物，单独或高压循环。通过基于液相色谱-高分辨率质谱法的特征分析，在正负电离模式下使用所有离子高能碰撞解离碎片，对浸提液进行评估。这种方法提供了类别特异性和脂质特异性片段，增强了脂质表征。溶剂基于疏水性优先提取脂质。更极性的物质优选MTBE;更疏水的化合物优选DCM。压力循环差异增加了一些脂质的产量。该平台能够分析>500种完整的亲脂物质，其中超过300种脂质跨越早产儿粪便中鉴定的6种LIPID MAPS类别。以前没有报告提供这些数据;因此，这项研究代表了评估弱势群体营养健康，炎症和传染病的新范式。
Robust methodologies for the analysis of fecal material will facilitate the understanding of gut (patho)physiology, its role(s) in health and disease and help improve care for individual patients, especially high-risk populations such as premature infants. Because lipidomics offers a biologically and analytically attractive approach, we developed a simple, sensitive, and quantitatively precise method for profiling intact lipids in fecal material. The method utilizes two separate, complementary extraction chemistries, dichloromethane (DCM) and a methyl tert-butyl ether/hexafluoroisopropanol (MTBE) mixture, alone or with high pressure cycling. Extracts were assessed by liquid chromatography-high resolution mass spectrometry-based profiling with all ion higher energy collisional dissociation fragmentation in both positive and negative ionization modes. This approach provides both class-specific and lipid-specific fragments, enhancing lipid characterization. Solvents preferentially extracted lipids based on hydrophobicity. More polar species preferred MTBE; more hydrophobic compounds preferred DCM. Pressure cycling differentially increased the yield of some lipids. The platform enabled analysis of >500 intact lipophillic species with over 300 lipids spanning 6 LIPID MAPS categories identified in the fecal matter from premature infants. No previous report exists that provides these data; thus, this study represents a new paradigm for assessing nutritional health, inflammation, and infectious disease in vulnerable populations.
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影响因子：
7.4
作者：
Bird, Susan S.;Marur, Vasant R.;Sniatynski, Matthew J.;Greenberg, Heather K.;Kristal, Bruce S.
通讯作者：
Kristal, Bruce S.
影响因子：
4.5
作者：
Hawkins, NJ;Bariol, C;Ward, RL
通讯作者：
Ward, RL
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
4.4
作者：
Hu, Chunxiu;van Dommelen, Judith;Hankemeier, Thomas
通讯作者：
Hankemeier, Thomas
影响因子：
1.9
作者：
Iverson, SJ;Lang, SLC;Cooper, MH
通讯作者：
Cooper, MH