Inhalable Thioflavin S for the Detection of Amyloid Beta Deposits in the Retina.
Inhalable Thioflavin S for the Detection of Amyloid Beta Deposits in the Retina.
复制标题
可吸入硫磺素S用于检测视网膜中的淀粉样蛋白β沉积。
DOI：
10.3390/molecules26040835
复制
发表时间：
2021-02-05
期刊：
影响因子：
--
通讯作者：
Pham W
中科院分区：
文献类型：
--
作者：
Barton SM;To E;Rogers BP;Whitmore C;Uppal M;Matsubara JA;Pham W
我们提出了一种综合递送技术，使用雾化方法将硫代黄素S用于成像视网膜中的淀粉样β蛋白(Abeta)沉积，作为大脑中Abeta的替代品，用于早期发现阿尔茨海默病。数据显示，野生型(WT)小鼠的视网膜中也有Abeta沉积，尽管比5XFAD小鼠少得多。此外，只有在5XFAD小鼠中，在整体和横断面数据中发现显著的Abeta沉积与视网膜神经节细胞(RGC)相关。此外，硫代黄素S所描绘的荧光信号与Abeta免疫组织化学染色信息相印证。总体而言，这种通过吸入的方法传递的探针也适用于其他Abeta结合分子，如刚果红、姜黄素和硫黄素T。与脑对应物相比，成像视网膜淀粉样蛋白沉积的优势在于，通过体内成像很容易接触到眼睛，并且它减少了设计必须穿过强大的血脑屏障的探针的工作量。
We present an integrated delivery technology herein employing the aerosolized method to repurpose thioflavin S for imaging amyloid beta (Abeta) deposits in the retina as a surrogate of Abeta in the brain for early detection of Alzheimer’s disease. The data showed that wild type (WT) mice also have Abeta deposits in the retinae, albeit much less than 5XFAD mice. Further, only in 5XFAD mice, significant Abeta deposits were found associated with retinal ganglion cells (RGCs) in whole-mount and cross-section data. Furthermore, the fluorescent signal depicted from thioflavin S corroborates with Abeta immunohistochemistry staining information. Overall, this probe delivery via inhalation method is also applicable to other Abeta-binding molecules, such as Congo red, curcumin, and thioflavin T. The advantage of imaging retinal amyloid deposits compared to the brain counterparts is that the eye is easily accessible by in vivo imaging and it reduces the effort to design a probe that must cross the formidable blood-brain barrier.
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DOI：
10.3233/jad-160289
发表时间：
2017
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
--
作者：
McClure R;Ong H;Janve V;Barton S;Zhu M;Li B;Dawes M;Jerome WG;Anderson A;Massion P;Gore JC;Pham W
通讯作者：
Pham W
影响因子：
5.7
作者：
Aoki, I;Wu, YJL;Koretsky, AP
通讯作者：
Koretsky, AP
DOI：
10.3233/jad-140798
发表时间：
2015
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
--
作者：
McClure R;Yanagisawa D;Stec D;Abdollahian D;Koktysh D;Xhillari D;Jaeger R;Stanwood G;Chekmenev E;Tooyama I;Gore JC;Pham W
通讯作者：
Pham W
DOI：
10.3791/51018
发表时间：
2013-12-13
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Ivanova E;Toychiev AH;Yee CW;Sagdullaev BT
通讯作者：
Sagdullaev BT
影响因子：
25
作者：
Higuchi, M;Iwata, N;Saido, TC
通讯作者：
Saido, TC