Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4 in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein.
Enhanced Expression of Sodium Hydrogen Exchanger (NHE)-1, 2 and 4 in the Cervix of Ovariectomised Rats by Phytoestrogen Genistein.
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DOI：
10.7150/ijms.11210
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发表时间：
2015
影响因子：
3.6
通讯作者：
Salleh N
中科院分区：
文献类型：
--
作者：
Ismail N;Giribabu N;Muniandy S;Salleh N
恢复宫颈阴道液的pH值对绝经后的宫颈阴道健康至关重要。金雀异黄素，这是一种广泛消费的饮食保健补充剂，以克服绝经后并发症可能有助于恢复宫颈阴道液pH值。我们假设，金雀异黄素的效果涉及的NHE-1，2和4蛋白和mRNA的表达在宫颈的变化。本研究探讨了染料木黄酮对NHE-1、2和4蛋白及mRNA在子宫颈中表达的影响，以阐明该化合物对绝经后子宫颈阴道液pH值的可能作用机制。方法：切除卵巢的成年雌性大鼠连续7天接受25、50和100 mg/kg/天的染料木黄酮。在处理结束时，处死动物并收获子宫颈。采用Real-time PCR检测NHE-1、2、4 mRNA的表达，免疫组化观察NHE-1、2、4蛋白的分布。结果如下：50和100 mg/kg/天染料木黄酮治疗引起宫颈内上皮中NHE-1、2和4蛋白的表达和分布水平显著增加。子宫颈Nhe-1、2和4 mRNA水平也升高。ICI 182 - 780和染料木黄酮联合应用可降低宫颈NHE-1、2和4蛋白及mRNA的表达水平。结论：在金雀异黄素的影响下，NHE-1，2和4蛋白和mRNA表达的增强可能有助于恢复宫颈阴道液的pH值，这可能有助于预防与绝经相关的宫颈阴道并发症。
Restoring the pH of cervicovaginal fluid is important for the cervicovaginal health after menopause. Genistein, which is a widely consumed dietary health supplement to overcome the post-menopausal complications could help to restore the cervicovaginal fluid pH. We hypothesized that genistien effect involves changes in expression of NHE-1, 2 and 4 proteins and mRNAs in the cervix. This study investigated effect of genistein on NHE-1, 2 and 4 protein and mRNA expression in the cervix in order to elucidate the mechanisms underlying possible effect of this compound on cervicovaginal fluid pH after menopause. Methods: Ovariectomised adult female rats received 25, 50 and 100 mg/kg/day genistein for seven consecutive days. At the end of the treatment, animals were sacrificed and cervix was harvested. Expression of Nhe-1, 2 and 4 mRNA were analyzed by Real-time PCR while distribution of NHE-1, 2 and 4 protein were observed by immunohistochemistry. Results: Treatment with 50 and 100 mg/kg/day genistein caused marked increase in the levels of expression and distribution of NHE-1, 2 and 4 proteins in the endocervical epithelia. Levels of Nhe-1, 2 and 4 mRNA in the cervix were also increased. Coadministration of ICI 182 780 and genistein reduced the expression levels of NHE-1, 2 and 4 proteins and mRNAs in the cervix. Conclusions: Enhanced expression of NHE-1, 2 and 4 proteins and mRNAs expression in cervix under genistein influence could help to restore the cervicovaginal fluid pH that might help to prevent cervicovaginal complications related to menopause.
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DOI：
10.1016/s1071-5576(96)00028-7
发表时间：
1996-09-01
期刊：
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
影响因子：
--
作者：
Gorodeski, GI
通讯作者：
Gorodeski, GI
影响因子：
--
作者：
GOOD, DW
通讯作者：
GOOD, DW
DOI：
10.1097/01.gme.0000109314.11228.e5
发表时间：
2004-07-01
影响因子：
2.7
作者：
Crisafulli, A;Marini, H;Squadrito, F
通讯作者：
Squadrito, F
影响因子：
5.6
作者：
Chinigarzadeh A;Kasim NF;Muniandy S;Kassim NM;Salleh N
通讯作者：
Salleh N
DOI：
10.1111/j.1749-6632.2009.04891.x
发表时间：
2009-01-01
期刊：
NATURAL COMPOUNDS AND THEIR ROLE IN APOPTOTIC CELL SIGNALING PATHWAYS
影响因子：
--
作者：
No, Jae Hong;Jo, Hoenil;Song, Yong-Sang
通讯作者：
Song, Yong-Sang