Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature.
Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature.
复制标题
DOI：
10.3390/ijms222111554
复制
发表时间：
2021-10-26
影响因子：
5.6
通讯作者：
Lundgaard I
中科院分区：
文献类型：
--
作者：
Battistella R;Kritsilis M;Matuskova H;Haswell D;Cheng AX;Meissner A;Nedergaard M;Lundgaard I
血管系统对所有组织来说都是至关重要的，对其可视化的兴趣跨越了许多领域。许多不同的植物来源的凝集素被用于检测血管系统；然而，缺乏对不同凝集素和技术的标记效率进行直接比较的研究。在这项研究中，我们比较了三种凝集素：简单叶灰树凝集素B4(IB4)、小麦胚凝集素(WGA)和番茄凝集素(LEA)的标记效果。LEA凝集素在脑血管的组织学标记方面远优于IB4和WGA凝集素。在心内灌流期间注射高浓度的WGA凝集素也能达到类似的信噪比。凝集素也适用于标记其他组织中的血管构筑，包括脊髓、硬脑膜、心脏、骨骼肌、肾脏和肝脏组织。在未损伤的组织中，LEA凝集素与Tie2-EGFP报告小鼠和GLUT-1免疫组织化学一样准确地标记脑血管，验证了其特异性和敏感性。然而，在病理情况下，例如在中风中，LEA凝集素的敏感性急剧下降，限制了其在此类研究中的适用性。本工作可用于各种组织凝集素类型的选择和标记方法。
The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different lectins and techniques are lacking. In this study, we compared the labeling efficacy of three lectins: Griffonia simplicifolia isolectin B4 (IB4); wheat germ agglutinin (WGA), and Lycopersicon esculentum agglutinin (LEA). The LEA lectin was identified as being far superior to the IB4 and WGA lectins in histological labeling of blood vessels in brain sections. A similar signal-to-noise ratio was achieved with high concentrations of the WGA lectin injected during intracardial perfusion. Lectins were also suitable for labeling vasculature in other tissues, including spinal cord, dura mater, heart, skeletal muscle, kidney, and liver tissues. In uninjured tissues, the LEA lectin was as accurate as the Tie2–eGFP reporter mice and GLUT-1 immunohistochemistry for labeling the cerebral vasculature, validating its specificity and sensitivity. However, in pathological situations, e.g., in stroke, the sensitivity of the LEA lectin decreases dramatically, limiting its applicability in such studies. This work can be used for selecting the type of lectin and labeling method for various tissues.
登录
查看更多内容
影响因子：
2.5
作者：
Düllmann, J;Van Damme, EJM;Schumacher, U
通讯作者：
Schumacher, U
影响因子：
6.1
作者：
Choudhury GR;Ding S
通讯作者：
Ding S
影响因子：
11.8
作者：
Birdsey, Graeme M.;Shah, Aarti V.;Dufton, Neil;Reynolds, Louise E.;Almagro, Lourdes Osuna;Yang, Youwen;Aspalter, Irene M.;Khan, Samia T.;Mason, Justin C.;Dejana, Elisabetta;Goettgens, Berthold;Hodivala-Dilke, Kairbaan;Gerhardt, Holger;Adams, Ralf H.;Randi, Anna M.
通讯作者：
Randi, Anna M.
影响因子：
4.2
作者：
BLACK, JE;POLINSKY, M;GREENOUGH, WT
通讯作者：
GREENOUGH, WT
影响因子：
3.3
作者：
Boero, JA;Ascher, J;Woolsey, TA
通讯作者：
Woolsey, TA