Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics.
Discovery of novel xylosides in co-culture of basidiomycetes Trametes versicolor and Ganoderma applanatum by integrated metabolomics and bioinformatics.
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综合代谢组学和生物信息学在担子菌、栓菌和扁平灵芝共培养中发现新型木糖苷
DOI：
10.1038/srep33237
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发表时间：
2016-09-12
影响因子：
4.6
通讯作者：
Yang S
中科院分区：
文献类型：
--
作者：
Yao L;Zhu LP;Xu XY;Tan LL;Sadilek M;Fan H;Hu B;Shen XT;Yang J;Qiao B;Yang S
对培养真菌的转录组学分析表明，在标准实验室条件下，许多次级代谢物合成基因可能是沉默的。为了研究共生系统中沉默基因的表达，将17种担子菌共培养，构建了136个真菌-真菌共生系统，其中杂色栓菌与树舌菌共培养的对峙区着色最强。代谢组学研究发现，与单独培养物相比，共培养物中有62个特征是新合成的或高度产生的。分子网络分析突出了包括两个新的木糖苷（化合物2和3）的子网络。化合物2经进一步鉴定为N-（4-甲氧基苯基）甲酰胺2-O-β-D-木糖苷，并显示其具有提高人永生化支气管上皮细胞系Beas-2B细胞活力的潜力。此外，还对T. versicolor揭示了编码木糖基化的木糖基转移酶的潜在候选基因（GI：636605689）。此外，还首次在G. applanatum，这可能归因于对T.versicolor胁迫的响应。总的来说，所描述的共培养平台提供了一个强大的工具来发现新的代谢物，并帮助深入了解真菌防御中沉默基因激活的机制。
Transcriptomic analysis of cultured fungi suggests that many genes for secondary metabolite synthesis are presumably silent under standard laboratory condition. In order to investigate the expression of silent genes in symbiotic systems, 136 fungi-fungi symbiotic systems were built up by co-culturing seventeen basidiomycetes, among which the co-culture of Trametes versicolor and Ganoderma applanatum demonstrated the strongest coloration of confrontation zones. Metabolomics study of this co-culture discovered that sixty-two features were either newly synthesized or highly produced in the co-culture compared with individual cultures. Molecular network analysis highlighted a subnetwork including two novel xylosides (compounds 2 and 3). Compound 2 was further identified as N-(4-methoxyphenyl)formamide 2-O-β-D-xyloside and was revealed to have the potential to enhance the cell viability of human immortalized bronchial epithelial cell line of Beas-2B. Moreover, bioinformatics and transcriptional analysis of T. versicolor revealed a potential candidate gene (GI: 636605689) encoding xylosyltransferases for xylosylation. Additionally, 3-phenyllactic acid and orsellinic acid were detected for the first time in G. applanatum, which may be ascribed to response against T.versicolor stress. In general, the described co-culture platform provides a powerful tool to discover novel metabolites and help gain insights into the mechanism of silent gene activation in fungal defense.
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影响因子：
5.2
作者：
Gressler M;Hortschansky P;Geib E;Brock M
通讯作者：
Brock M
影响因子：
2.5
作者：
Eyre, Catherine;Muftah, Wafa;Rogers, Hilary J.
通讯作者：
Rogers, Hilary J.
影响因子：
--
作者：
Holman, Jerry D;Tabb, David L;Mallick, Parag
通讯作者：
Mallick, Parag
影响因子：
3
作者：
Chen Qi-he;Kruegener, Sven;Zibek, Susanne
通讯作者：
Zibek, Susanne
影响因子：
2.2
作者：
Fonseca, María Isabel;Tejerina, Marcos Raúl;Villalba, Laura Lidia
通讯作者：
Villalba, Laura Lidia