Quantitative analysis of viral load per haploid genome revealed the different biological features of Merkel cell polyomavirus infection in skin tumor.
Quantitative analysis of viral load per haploid genome revealed the different biological features of Merkel cell polyomavirus infection in skin tumor.
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对每个单倍体基因组病毒负荷的定量分析揭示了皮肤肿瘤中默克尔细胞多瘤病毒感染的不同生物学特征。
DOI：
10.1371/journal.pone.0039954
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fukayama M
中科院分区：
文献类型：
--
作者：
Ota S;Ishikawa S;Takazawa Y;Goto A;Fujii T;Ohashi K;Fukayama M
最近在默克尔细胞癌（MCC）中发现了默克尔细胞多瘤病毒（MCPyV），这是一种发生在暴露于阳光下的皮肤中的侵袭性癌症。传统的技术，如聚合酶链反应（PCR）和免疫组织化学，产生了相互矛盾的结果MCPyV感染非MCC肿瘤。因此，我们对各种皮肤肿瘤组织中的MCPyV拷贝数进行了定量分析，包括MCC（n = 9）和其他日光照射相关的皮肤肿瘤（基底细胞癌[BCC，n = 45]、光化性角化病[AK，n = 52]、Bowen病[n = 34]、脂溢性角化病[n = 5]、原发性皮肤间变性大细胞淋巴瘤[n = 5]、恶性黑色素瘤[n = 5]和黑色素细胞痣[n = 6]）。 在常规PCR分析中，在MCC（9例; 100%）、BCC（1例; 2%）和AK（3例; 6%）中检测到MCPyV DNA。然后，我们使用数字PCR技术来估计这些组织中每个单倍体人类基因组的绝对病毒拷贝数。在大多数MCC组织中，每个单倍体基因组的病毒拷贝数估计为1左右，MCC组（0.119-42.8）和AK组（0.02-0.07）之间存在显著差异。PCR阳性BCC组织显示与MCC组织相似的病毒载量（0.662）。免疫组织化学与MCPyV T抗原（CM 2B 4）的单克隆抗体显示阳性核定位在大多数高病毒载量肿瘤组（8/9 MCC和1 BCC），但不是在低病毒载量或PCR阴性肿瘤组。这些结果表明，MCPyV感染可能涉及少数暴露于阳光的皮肤肿瘤，包括BCC和AK，并且这些肿瘤显示不同的感染模式。
Merkel cell polyomavirus (MCPyV) has recently been identified in Merkel cell carcinoma (MCC), an aggressive cancer that occurs in sun-exposed skin. Conventional technologies, such as polymerase chain reaction (PCR) and immunohistochemistry, have produced conflicting results for MCPyV infections in non-MCC tumors. Therefore, we performed quantitative analyses of the MCPyV copy number in various skin tumor tissues, including MCC (n = 9) and other sun exposure-related skin tumors (basal cell carcinoma [BCC, n = 45], actinic keratosis [AK, n = 52], Bowen’s disease [n = 34], seborrheic keratosis [n = 5], primary cutaneous anaplastic large-cell lymphoma [n = 5], malignant melanoma [n = 5], and melanocytic nevus [n = 6]). In a conventional PCR analysis, MCPyV DNA was detected in MCC (9 cases; 100%), BCC (1 case; 2%), and AK (3 cases; 6%). We then used digital PCR technology to estimate the absolute viral copy number per haploid human genome in these tissues. The viral copy number per haploid genome was estimated to be around 1 in most MCC tissues, and there were marked differences between the MCC (0.119–42.8) and AK (0.02–0.07) groups. PCR-positive BCC tissue showed a similar viral load as MCC tissue (0.662). Immunohistochemistry with a monoclonal antibody against the MCPyV T antigen (CM2B4) demonstrated positive nuclear localization in most of the high-viral-load tumor groups (8 of 9 MCC and 1 BCC), but not in the low-viral-load or PCR-negative tumor groups. These results demonstrated that MCPyV infection is possibly involved in a minority of sun-exposed skin tumors, including BCC and AK, and that these tumors display different modes of infection.
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影响因子：
5.3
作者：
MANOS, MM;GLUZMAN, Y
通讯作者：
GLUZMAN, Y
影响因子：
1.7
作者：
Andres, Christian;Belloni, Benedetta;Flaig, Michael J.
通讯作者：
Flaig, Michael J.
影响因子：
12.7
作者：
Katano, Harutaka;Ito, Hideki;Sata, Tetsutaro
通讯作者：
Sata, Tetsutaro
DOI：
10.1097/pas.0b013e3181aa30a5
发表时间：
2009-09
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Busam KJ;Jungbluth AA;Rekthman N;Coit D;Pulitzer M;Bini J;Arora R;Hanson NC;Tassello JA;Frosina D;Moore P;Chang Y
通讯作者：
Chang Y
影响因子：
6.4
作者：
Loyo, Myriam;Guerrero-Preston, Rafael;Brait, Mariana;Hoque, Mohmammad O.;Chuang, Alice;Kim, Myoung S.;Sharma, Rajni;Liegeois, Nanette J.;Koch, Wayne M.;Califano, Joseph A.;Westra, William H.;Sidransky, David
通讯作者：
Sidransky, David