Enriched expression of serotonin 1B and 2A receptor genes in macaque visual cortex and their bidirectional modulatory effects on neuronal responses.
Enriched expression of serotonin 1B and 2A receptor genes in macaque visual cortex and their bidirectional modulatory effects on neuronal responses.
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DOI：
10.1093/cercor/bhn219
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Yamamori T
中科院分区：
文献类型：
--
作者：
Watakabe A;Komatsu Y;Sadakane O;Shimegi S;Takahata T;Higo N;Tochitani S;Hashikawa T;Naito T;Osaki H;Sakamoto H;Okamoto M;Ishikawa A;Hara S;Akasaki T;Sato H;Yamamori T
为了研究成年灵长类大脑皮层专化区的分子机制，我们搜索了猕猴的区域特异性基因，发现5-羟色胺(5-羟色胺，5-羟色胺)1B受体mRNA在初级视区(V1)显著丰富，5-HT2A受体mRNA的丰度较小。原位杂交分析表明，这两种基因高度集中在膝状体始层IVA和IVC，它们在相同的神经元中共表达。单眼注射河豚毒素灭活导致这些mRNAs强烈而迅速的下调，提示它们的表达对视网膜活动有依赖性。在体电生理实验中，5-HT1B和5-HT2A受体激动剂对V1神经元的反应有明显的调制作用，这与V1神经元的高表达水平一致。5-HT1B激动剂的调制作用依赖于记录到的神经元的放电频率：对高放电频率的神经元有促进作用，对低放电频率的神经元有抑制作用。5-HT2A激动剂则表现出相反的作用。这些结果表明，这个5-羟色胺能系统控制V1的视觉反应，以优化对传入视觉输入的信息处理。
To study the molecular mechanism how cortical areas are specialized in adult primates, we searched for area-specific genes in macaque monkeys and found striking enrichment of serotonin (5-hydroxytryptamine, 5-HT) 1B receptor mRNA, and to a lesser extent, of 5-HT2A receptor mRNA, in the primary visual area (V1). In situ hybridization analyses revealed that both mRNA species were highly concentrated in the geniculorecipient layers IVA and IVC, where they were coexpressed in the same neurons. Monocular inactivation by tetrodotoxin injection resulted in a strong and rapid (<3 h) downregulation of these mRNAs, suggesting the retinal activity dependency of their expression. Consistent with the high expression level in V1, clear modulatory effects of 5-HT1B and 5-HT2A receptor agonists on the responses of V1 neurons were observed in in vivo electrophysiological experiments. The modulatory effect of the 5-HT1B agonist was dependent on the firing rate of the recorded neurons: The effect tended to be facilitative for neurons with a high firing rate, and suppressive for those with a low firing rate. The 5-HT2A agonist showed opposite effects. These results suggest that this serotonergic system controls the visual response in V1 for optimization of information processing toward the incoming visual inputs.
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影响因子：
2.9
作者：
Guzmán-Marín, R;Alam, MN;McGinty, D
通讯作者：
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影响因子：
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影响因子：
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影响因子：
4.7
作者：
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PALACIOS, JM