Resolution improvement by 3D particle averaging in localization microscopy.
Resolution improvement by 3D particle averaging in localization microscopy.
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DOI：
10.1088/2050-6120/3/1/014003
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发表时间：
2015-03
影响因子：
3.2
通讯作者：
Rieger B
中科院分区：
文献类型：
--
作者：
Broeken J;Johnson H;Lidke DS;Liu S;Nieuwenhuizen RP;Stallinga S;Lidke KA;Rieger B
受定位显微镜的最新发展的启发，在二维中应用相同粒子的平均以进一步提高分辨率，我们讨论了一般和特别是3D粒子的对准（配准）方法。我们详细说明了传统的基于互相关的低温电子显微镜粒子配准技术是不合适的，因为底层的图像形成过程是根本不同的。我们认为，只有局域化，即一组坐标与相关的不确定性，被记录，而不是一个连续的强度分布。我们提出了一种基于这一事实的方法，并受到统计模式识别领域的启发。我们特别建议使用巴塔查里亚距离的改编版本作为注册的功绩函数。我们在模拟中评估了该方法，并在Hela细胞核孔复合物中标记Nup133蛋白的Alexa 647的三维超分辨率数据上进行了验证。从模拟中我们发现，为了成功配准，定位不确定性必须小于粒子上标记点之间的距离。这些建议得到了关于定位显微镜可达到的分辨率及其标度行为作为标记密度和定位精度函数的理论考虑的支持。
Inspired by recent developments in localization microscopy that applied averaging of identical particles in 2D for increasing the resolution even further, we discuss considerations for alignment (registration) methods for particles in general and for 3D in particular. We detail that traditional techniques for particle registration from cryo electron microscopy based on cross-correlation are not suitable, as the underlying image formation process is fundamentally different. We argue that only localizations, i.e. a set of coordinates with associated uncertainties, are recorded and not a continuous intensity distribution. We present a method that owes to this fact and that is inspired by the field of statistical pattern recognition. In particular we suggest to use an adapted version of the Bhattacharyya distance as a merit function for registration. We evaluate the method in simulations and demonstrate it on three-dimensional super-resolution data of Alexa 647 labelled to the Nup133 protein in the nuclear pore complex of Hela cells. From the simulations we find suggestions that for successful registration the localization uncertainty must be smaller than the distance between labeling sites on a particle. These suggestions are supported by theoretical considerations concerning the attainable resolution in localization microscopy and its scaling behavior as a function of labeling density and localization precision.
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影响因子：
48
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
DOI：
10.1016/j.str.2010.05.008
发表时间：
2010-07-14
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Liao HY;Frank J
通讯作者：
Frank J
影响因子：
6.1
作者：
Frank, Joachim
通讯作者：
Frank, Joachim
影响因子：
16.6
作者：
Ehmann, Nadine;van de Linde, Sebastian;Alon, Amit;Ljaschenko, Dmitrij;Keung, Xi Zhen;Holm, Thorge;Rings, Annika;DiAntonio, Aaron;Hallermann, Stefan;Ashery, Uri;Heckmann, Manfred;Sauer, Markus;Kittel, Robert J.
通讯作者：
Kittel, Robert J.
影响因子：
6.8
作者：
Briggs, John A. G.
通讯作者：
Briggs, John A. G.