Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress.
Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress.
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褪黑素对干旱胁迫下裸燕麦苗抗氧化能力的影响
DOI：
10.3390/molecules23071580
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发表时间：
2018-06-29
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Gao W;Zhang Y;Feng Z;Bai Q;He J;Wang Y
褪黑素（n -乙酰基-5-甲氧基色胺，MT）是一种具有多种作用的分子，包括抗氧化活性，调节植物生长发育和减轻植物的环境胁迫。然而，外源MT对裸燕麦幼苗干旱胁迫影响的研究较少。因此，在本研究中，我们研究了外源MT对干旱胁迫下裸燕麦抗氧化能力的影响，了解可能的抗氧化机制。结果表明，100 μM MT预处理能降低过氧化氢（H2O2）和超氧阴离子（O2−•）的含量。MT还提高了20% PEG-6000干旱胁迫下裸燕麦幼苗叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性。MT上调了丝裂原活化蛋白激酶（MAPKs） Asmap1和Aspk11的表达水平，转录因子（TF）基因（除NAC外）、WRKY1、DREB2和MYB在干旱胁迫下处理的幼苗中表达水平高于单独处理的幼苗。这些数据表明，mt介导的抗氧化反应的诱导可能需要激活活性氧（ROS）和MAPK，随后触发下游MAPK级联，如Asmap1和Aspk11，以调节抗氧化相关基因的表达。本研究表明，MT可以诱导MAPKs和TFs的表达，调控下游胁迫响应基因的表达，从而提高植物的耐受性。这可能为MT调控植物抗氧化防御提供新的思路。这些结果为裸麦秸秆移植缓解干旱胁迫提供了理论依据。
Melatonin (N-acetyl-5-methoxytryptamine, MT) is a molecule with pleiotropic effects including antioxidant activity, regulated plant growth, development, and reduced environmental stress in plants. However, only a few studies have analyzed the effect of exogenous MT on drought stress in naked oat seedlings. Therefore, in this study, we studied the effects of exogenous MT on the antioxidant capacity of naked oat under drought stress to understand the possible antioxidant mechanism. The results showed that a pretreatment of 100 μM MT reduced the hydrogen peroxide (H2O2) and superoxide anion (O2−•) contents. MT also enhanced superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) activities in the leaves of naked oat seedlings under 20% PEG-6000 drought stress. MT upregulated the expression levels of the mitogen-activated protein kinases (MAPKs) Asmap1 and Aspk11, and the transcription factor (TF) genes (except for NAC), WRKY1, DREB2, and MYB increased in drought with MT pretreatment seedlings when compared with seedlings exposed to drought stress alone. These data indicated that the MT-mediated induction of the antioxidant response may require the activation of reactive oxygen species (ROS) and MAPK, followed by triggering a downstream MAPK cascade such as Asmap1 and Aspk11, to regulate the expression of antioxidant-related genes. This study demonstrated that MT could induce the expression of MAPKs and TFs and regulate the expression of downstream stress-responsive genes, thereby increasing the plant’s tolerance. This may provide a new idea for MT modulation in the regulation of plant antioxidant defenses. These results provide a theoretical basis for MT to alleviate drought stress in naked oat.
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影响因子：
10.3
作者：
DUBBELS, R;REITER, RJ;SCHLOOT, W
通讯作者：
SCHLOOT, W
影响因子：
10.3
作者：
Arnao, M. B.;Hernandez-Ruiz, J.
通讯作者：
Hernandez-Ruiz, J.
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
Farooq, M.;Wahid, A.;Basra, S. M. A.
通讯作者：
Basra, S. M. A.
水杨酸对小麦幼苗抗氧化系统和光系统II的影响
影响因子：
1.5
作者：
Chen, Y. E.;Cui, J. M.;Zhang, H. Y.
通讯作者：
Zhang, H. Y.
影响因子：
64.8
作者：
Asai, T;Tena, G;Sheen, J
通讯作者：
Sheen, J