Spatial and developmental heterogeneity of calcium signaling in olfactory ensheathing cells
Spatial and developmental heterogeneity of calcium signaling in olfactory ensheathing cells
复制标题
嗅鞘细胞中钙信号的空间和发育异质性
作者：
Thyssen A;Stavermann M;Buddrus K;Doengi M;Ekberg JA;St John JA;Deitmer JW;Lohr C
嗅鞘细胞（OECs）是哺乳动物嗅觉系统中支持轴突从嗅上皮向嗅球生长的特化胶质细胞。根据标记蛋白的表达及其在神经层的位置，嗅球中的oec可分为外神经层oec和内神经层oec。在本研究中，我们使用了急性小鼠脑切片OEC和tototo嗅球的共聚焦钙成像来研究OEC亚群之间的生理差异。外神经层的oec对谷氨酸、ATP、5 -羟色胺、多巴胺、氨基酚和苯肾上腺素有反应，胞质钙浓度升高。钙反应包括短暂性和补补性成分，后者由储存操作的钙进入介导。在出生后的前三周，oec中的钙测量显示，mglur1和p2y1受体介导的钙信号在前两周内下调，这表明这些受体的表达受到发育控制。此外，电刺激感觉轴突仅在外神经层oec中通过mglur1和p2y1激活钙信号。出生后动物受体介导的钙反应的下调反映在出生后3至21天oec中刺激引起的钙瞬变幅度的下降。总之，这些结果揭示了嗅球中两个oec亚群在发育过程中的受体反应和轴突- oec通讯方面的显著差异。©2012 Wiley期刊公司
Olfactory ensheathing cells (OECs) are specialized glial cells in the mammalian olfactory system supporting growth of axons from the olfactory epithelium into the olfactory bulb. OECs in the olfactory bulb can be subdivided into OECs of the outer nerve layer and the inner nerve layer according to the expression of marker proteins and their location in the nerve layer. In the present study, we have used confocal calcium imaging of OECs in acute mouse brain slices and olfactory bulbsin tototo investigate physiological differences between OEC subpopulations. OECs in the outer nerve layer, but not the inner nerve layer, responded to glutamate, ATP, serotonin, dopamine, carbachol, and phenylephrine with increases in the cytosolic calcium concentration. The calcium responses consisted of a transient and a tonic component, the latter being mediated by store‐operated calcium entry. Calcium measurements in OECs during the first three postnatal weeks revealed a downregulation of mGluR1and P2Y1receptor‐mediated calcium signaling within the first 2 weeks, suggesting that the expression of these receptors is developmentally controlled. In addition, electrical stimulation of sensory axons evoked calcium signaling via mGluR1and P2Y1only in outer nerve layer OECs. Downregulation of the receptor‐mediated calcium responses in postnatal animals is reflected by a decrease in amplitude of stimulation‐evoked calcium transients in OECs from postnatal days 3 to 21. In summary, the results presented reveal striking differences in receptor responses during development and in axon–OEC communication between the two subpopulations of OECs in the olfactory bulb. © 2012 Wiley Periodicals, Inc.
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