MARGO (Massively Automated Real-time GUI for Object-tracking), a platform for high-throughput ethology

MARGO (Massively Automated Real-time GUI for Object-tracking), a platform for high-throughput ethology
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MARGO(用于对象跟踪的大规模自动化实时 GUI),高通量行为学平台

DOI:
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发表时间:
2019
期刊:
bioRxiv
影响因子:
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通讯作者:
Benjamin L. de Bivort
Benjamin L. de Bivort
中科院分区:
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文献类型:
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作者:
Zach Werkhoven;Christian Rohrsen;Chuan Qin;B. Brembs;Benjamin L. de Bivort

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真实的时间内的快速对象跟踪允许方便地跟踪非常大量的动物和并行控制多个动物的刺激的闭环实验。我们开发了MARGO,这是一个用于定制行为实验的实时动物跟踪套件。我们证明了MARGO可以在很长的时间尺度上快速准确地并行跟踪大量动物。我们结合了外围硬件的控制,并实现了一个灵活的软件架构,定义新的实验例程。这些特征使得能够同时向许多个体闭环递送刺激。我们强调MARGO的能力,协调跟踪和硬件控制与两个自定义行为测定(测量趋光性和optomotor反应)和一个光遗传操作性条件反射测定。目前有几个开源的动物追踪器。MARGO的优势在于:1)鲁棒性,2)高通量,3)灵活的硬件控制和4)感官和光遗传刺激的实时闭环控制,所有这些都是针对大规模实验而优化的。
Fast object tracking in real time allows convenient tracking of very large numbers of animals and closed-loop experiments that control stimuli for multiple animals in parallel. We developed MARGO, a real-time animal tracking suite for custom behavioral experiments. We demonstrated that MARGO can rapidly and accurately track large numbers of animals in parallel over very long timescales. We incorporated control of peripheral hardware, and implemented a flexible software architecture for defining new experimental routines. These features enable closed-loop delivery of stimuli to many individuals simultaneously. We highlight MARGO’s ability to coordinate tracking and hardware control with two custom behavioral assays (measuring phototaxis and optomotor response) and one optogenetic operant conditioning assay. There are currently several open source animal trackers. MARGO’s strengths are 1) robustness, 2) high throughput, 3) flexible control of hardware and 4) real-time closed-loop control of sensory and optogenetic stimuli, all of which are optimized for large-scale experimentation.
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