Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer.
Profiling the Urinary Microbiome in Men with Positive versus Negative Biopsies for Prostate Cancer.
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DOI：
10.1016/j.juro.2017.08.001
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Sfanos KS
中科院分区：
文献类型：
--
作者：
Shrestha E;White JR;Yu SH;Kulac I;Ertunc O;De Marzo AM;Yegnasubramanian S;Mangold LA;Partin AW;Sfanos KS
研究表明，尿样中的细菌DNA和可培养细菌对临床上认为尿液是无菌的教条提出了挑战。此外，现在的研究表明，尿路微生物群的失调与病理条件有关。我们认为，尿液微生物群可能影响前列腺癌的慢性炎症，从而导致前列腺癌的发生和发展。因此，我们对前列腺癌活检阳性和阴性的男性的尿菌群进行了分析。尿液是在前列腺癌活检前从男性身上收集的。从尿样中提取DNA，进行细菌16S rDNA Illumina®测序和16S rDNA定量聚合酶链式反应。我们确定了细菌种类与癌症的存在或不存在、癌症分级以及前列腺癌的类型和程度之间的关系。尿样显示出不同的细菌种群。在α或β多样性方面没有显著差异，良性肿瘤样本之间也没有明显的等级聚类。我们在一组样本中发现了一群以前与泌尿生殖道感染有关的促炎细菌。许多物种，包括已知的尿路病原体，在癌症和良性疾病样本中，在低级别和高级别癌症中显著和不同地丰富，并与前列腺癌的类型和程度有关。据我们所知，我们报道了迄今为止对男性尿路微生物组及其与前列腺癌的关系的最全面的研究。我们的结果表明前列腺癌患者的尿路中存在促炎性细菌和尿路病原体。
Studies demonstrating bacterial DNA and cultivable bacteria in urine samples have challenged the clinical dogma that urine is sterile. Furthermore, studies now indicate that dysbiosis of the urinary microbiome is associated with pathological conditions. We propose that the urinary microbiome may influence chronic inflammation observed in the prostate, leading to prostate cancer development and progression. Therefore, we profiled the urinary microbiome in men with positive vs negative biopsies for prostate cancer. Urine was collected from men prior to biopsy for prostate cancer. DNA was extracted from urine pellet samples and subjected to bacterial 16S rDNA Illumina® sequencing and 16S rDNA quantitative polymerase chain reaction. We determined the association between bacterial species and the presence or absence of cancer, cancer grade, and type and degree of prostate inflammation. Urine samples revealed diverse bacterial populations. There were no significant differences in α or β diversity and no clear hierarchical clustering of benign or cancer samples. We identified a cluster of pro-inflammatory bacteria previously implicated in urogenital infections in a subset of samples. Many species, including known uropathogens, were significantly and differentially abundant among cancer and benign samples, in low vs higher grade cancers and in relation to prostate inflammation type and degree. To our knowledge we report the most comprehensive study to date of the male urinary microbiome and its relationship to prostate cancer. Our results suggest a prevalence of pro-inflammatory bacteria and uropathogens in the urinary tract of men with prostate cancer.
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