Preconceptional diet manipulation and fetus number can influence placenta endocrine function in sheep.
Preconceptional diet manipulation and fetus number can influence placenta endocrine function in sheep.
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DOI：
10.1016/j.domaniend.2020.106577
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发表时间：
2021-01
影响因子：
2.1
通讯作者：
Veiga-Lopez A
中科院分区：
文献类型：
--
作者：
Rosales-Nieto CA;Ehrhardt R;Mantey A;Makela B;Byrem;Veiga-Lopez A
孕期母体营养的变化会对胎盘功能和胎儿发育产生深远的影响。虽然妊娠前期有可能改变胚胎和胎盘的发育，但关于配对前营养操作对胎盘功能以及胎儿和出生后后代生长的影响，我们知之甚少。为了验证这一点，将99只多赛特多赛特绵羊分配到三个营养处理之一(n=33/处理)，在4-5月交配前21天(增加光周期)，接受50%(UN：营养不良)、100%(C：对照)或200%(营养过剩)的维持能量需要量。此后，不同组的饮食都是相同的。我们通过断奶评估母体生殖变量和母子体重和体重指数。孕期至出生后第1天采集母体血浆，测定妊娠相关糖蛋白(PAG)和孕酮。不同处理之间的受精率相似，但雌性受精率较高(联合国：82%；C：100%：145%)。根据总出生体重进行校正后，双胎妊娠的平均动脉血糖值和孕酮水平低于单胎妊娠(P<；0.001)。出生时，无论出生类型如何，联合国羔羊都比C羔羊重(P<；0.01)。生长速度、日增重和断奶体重相似，但UN和On母鱼生长较快，且在断奶时比C母鱼重。我们证明，怀孕前3周的母亲营养不足或营养过剩，当根据总出生体重进行校正时，会导致双胎妊娠的内分泌能力降低。怀孕前母体营养不足和营养过剩增加了出生后雌性羔羊的生长，暗示着受孕前调节生长的途径重新编程。这突显了怀孕前的营养如何导致明显的性别差异。
Changes in maternal nutrition during pregnancy can result in profound effects on placental function and fetal development. Although the pre-conceptional period holds the potential to reprogram embryonic and placental development, little is known regarding the effects of pre-mating nutritional manipulation on placental function and fetal and postnatal offspring growth. To test this, Polypay-Dorset sheep (n=99) were assigned to one of three nutritional treatments (n=33/treatment) receiving 50% (UN: undernutrition), 100% (C: control), or 200% (ON: overnutrition) of maintenance energy requirements for 21 days before mating during April-May (increasing photoperiod). Thereafter, diets were the same across groups. We evaluated maternal reproductive variables and maternal and offspring weight and body mass index through weaning. Maternal plasma was collected through pregnancy until postnatal day 1 to assay pregnancy associated glycoproteins (PAG) and progesterone. Fertility rate was similar among treatments, but ON females had a higher reproductive rate (UN: 82%; C:100% ON:145%). When correcting by total birth weight, twin pregnancies had lower PAG and progesterone vs. singleton pregnancies (P<0.001). At birth, UN lambs were heavier than C lambs regardless of birth type (P<0.01). Growth velocity, daily gain, and weaning weight were similar, but UN and ON females grew faster and were heavier at weaning vs. C females. We demonstrated that a 3-week pre-conceptional maternal under- or overnutrition, when correcting by total birth weight, results in lower endocrine capacity in twin pregnancies. Pre-conceptional maternal under- and overnutrition increased postnatal female lamb growth, suggestive of reprogramming of pathways regulating growth before conception. This highlights how pre-conceptional nutrition can result in marked sex-specific differences.
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影响因子：
5.5
作者：
Fowden, AL;Ward, JW;Constancia, M
通讯作者：
Constancia, M
影响因子：
3.8
作者：
Ehrhardt, RA;Bell, AW
通讯作者：
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作者：
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通讯作者：
Szenci, O.
DOI：
10.1016/j.cppeds.2011.01.001
发表时间：
2011-07
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FRISCH, RE;MCARTHUR, JW
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MCARTHUR, JW