Administration of reconstituted polyphenol oil bodies efficiently suppresses dendritic cell inflammatory pathways and acute intestinal inflammation.
Administration of reconstituted polyphenol oil bodies efficiently suppresses dendritic cell inflammatory pathways and acute intestinal inflammation.
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DOI：
10.1371/journal.pone.0088898
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chieppa M
中科院分区：
文献类型：
--
作者：
Cavalcanti E;Vadrucci E;Delvecchio FR;Addabbo F;Bettini S;Liou R;Monsurrò V;Huang AY;Pizarro TT;Santino A;Chieppa M
多酚是一种天然化合物，能够干扰几种体外模型系统的炎症途径。在这项研究中，我们开发了一种稳定有效的策略，给药多酚治疗急性肠道炎症的体内模型。几种多酚的体外抑制特性首次测试和比较树突状细胞（DCs）的炎症细胞因子的生产。多酚、槲皮素和胡椒碱的组合，然后被封装到重组油体（OBs）中，以增加其稳定性。我们的研究结果表明，低剂量重组多酚ob抑制lps介导的炎症细胞因子分泌，包括IL-6、IL-23和IL-12，同时增加IL-10和IL-1Rα的产生。用含多酚的重组OBs （ROBs）治疗的小鼠部分免受葡聚糖硫酸钠（DSS）诱导的结肠炎和相关体重减轻的影响，而死亡率和炎症评分显示，总体上抗炎作用可能是由DC免疫反应受损介导的。我们的研究表明，重组槲皮素和含胡椒碱的OBs可能是治疗急性肠道炎症的有效和有效的抗炎策略。
Polyphenols are natural compounds capable of interfering with the inflammatory pathways of several in vitro model systems. In this study, we developed a stable and effective strategy to administer polyphenols to treat in vivo models of acute intestinal inflammation. The in vitro suppressive properties of several polyphenols were first tested and compared for dendritic cells (DCs) production of inflammatory cytokines. A combination of the polyphenols, quercetin and piperine, were then encapsulated into reconstituted oil bodies (OBs) in order to increase their stability. Our results showed that administration of low dose reconstituted polyphenol OBs inhibited LPS-mediated inflammatory cytokine secretion, including IL-6, IL-23, and IL-12, while increasing IL-10 and IL-1Rα production. Mice treated with the polyphenol-containing reconstituted OBs (ROBs) were partially protected from dextran sodium sulfate (DSS)-induced colitis and associated weight loss, while mortality and inflammatory scores revealed an overall anti-inflammatory effect that was likely mediated by impaired DC immune responses. Our study indicates that the administration of reconstituted quercetin and piperine-containing OBs may represent an effective and potent anti-inflammatory strategy to treat acute intestinal inflammation.
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影响因子：
6.7
作者：
Naito, Y;Takagi, T;Yoshikawa, T
通讯作者：
Yoshikawa, T
宿主防御途径：冗余和补偿在传染病表型中的作用。
影响因子：
32.4
作者：
Nish S;Medzhitov R
通讯作者：
Medzhitov R
影响因子：
4.1
作者：
Cai, X.;Fang, Z.;Zhai, G.
通讯作者：
Zhai, G.
影响因子：
5.4
作者：
Comalada, M;Camuesco, D;Zarzuelo, A
通讯作者：
Zarzuelo, A
影响因子：
3.9
作者：
Bettini, Simona;Vergara, Daniele;Santino, Angelo
通讯作者：
Santino, Angelo