Unidirectional P-body transport during the yeast cell cycle.
Unidirectional P-body transport during the yeast cell cycle.
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DOI：
10.1371/journal.pone.0099428
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Dudley AM
中科院分区：
文献类型：
--
作者：
Garmendia-Torres C;Skupin A;Michael SA;Ruusuvuori P;Kuwada NJ;Falconnet D;Cary GA;Hansen C;Wiggins PA;Dudley AM
P-体属于 RNA 颗粒大家族，与转录后基因调控相关，从酵母到哺乳动物都是保守的，并影响从生殖细胞发育到神经元可塑性的生物过程。 RNA 颗粒还可以将 RNA 运输到特定位置。胚芽颗粒将母体 RNA 转运至胚胎，神经元颗粒将 RNA 长距离转运至突触树突。在这里，我们结合基于微流体的单细胞荧光显微镜和自动图像分析来跟踪酵母细胞分裂过程中的 p 体动力学。我们的结果表明，这些高度动态的颗粒在有丝分裂期间经历从母细胞到子细胞的单向运输，并且在可观察到芽之前半小时在芽位置附近受限“盘旋”。这两种行为都依赖于 Myo4p/She2p RNA 转运机制。此外，细胞大小的单细胞分析表明，PBs 在营养限制条件下的子细胞生长中发挥着重要作用。
P-bodies belong to a large family of RNA granules that are associated with post-transcriptional gene regulation, conserved from yeast to mammals, and influence biological processes ranging from germ cell development to neuronal plasticity. RNA granules can also transport RNAs to specific locations. Germ granules transport maternal RNAs to the embryo, and neuronal granules transport RNAs long distances to the synaptic dendrites. Here we combine microfluidic-based fluorescent microscopy of single cells and automated image analysis to follow p-body dynamics during cell division in yeast. Our results demonstrate that these highly dynamic granules undergo a unidirectional transport from the mother to the daughter cell during mitosis as well as a constrained “hovering” near the bud site half an hour before the bud is observable. Both behaviors are dependent on the Myo4p/She2p RNA transport machinery. Furthermore, single cell analysis of cell size suggests that PBs play an important role in daughter cell growth under nutrient limiting conditions.
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影响因子：
48
作者：
Lubeck, Eric;Cai, Long
通讯作者：
Cai, Long
影响因子：
30.8
作者：
Delneri, Daniela;Hoyle, David C.;Oliver, Stephen G.
通讯作者：
Oliver, Stephen G.
影响因子：
5.3
作者：
Desnos, Claire;Huet, Sebastien;Darchen, Francois
通讯作者：
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影响因子：
4.1
作者：
Iwaki, Aya;Izawa, Shingo
通讯作者：
Izawa, Shingo
mRNA 脱帽和 P 体形成的控制。
影响因子：
16
作者：
Franks, Tobias M.;Lykke-Andersen, Jens
通讯作者：
Lykke-Andersen, Jens