Tag-encoded pyrosequencing analysis of bacterial diversity within different alpine grassland ecosystems of the Qinghai-Tibet Plateau, China
Tag-encoded pyrosequencing analysis of bacterial diversity within different alpine grassland ecosystems of the Qinghai-Tibet Plateau, China
复制标题
青藏高原不同高寒草原生态系统细菌多样性的标签编码焦磷酸测序分析
DOI：
10.1007/s12665-013-3001-z
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发表时间：
2013
影响因子：
2.8
通讯作者：
Dyson, Paul J.
中科院分区：
文献类型：
--
作者：
Dong, Zhibao;Zhang, Gaosen;Chen, Tuo;Dyson, Paul J.
青藏高原对气候变化敏感，生态系统对科学研究具有重要意义。中国，高通量DNA焦磷酸测序被用来评估青藏高原不同高寒草原生态系统中的细菌多样性。共获得高寒沼泽草甸(ASM)、高寒草甸(AM)、高寒沙质草原(ASG)三个生态系统的34,759个序列，共检测到31个门和少量未分类细菌。不同高寒草地生态系统的细菌群落结构不同。变形杆菌和酸性细菌是三个生态系统中的优势类群。此外，ASM中还含有大量的放线菌和氯杆菌，AM中有大量的拟杆菌、双歧杆菌和微囊藻，ASG中有大量的放线菌。此外，不同高寒草地生态系统的功能细菌属也不同。ASM含有更多的硝化细菌、甲烷氧化菌和硫酸盐还原细菌，而ASG生态系统含有更多的固氮菌。焦磷酸测序提供了比以前更多的关于不同高寒草原生态系统中细菌多样性的洞察，并为细菌参与青藏高原高寒草原生态系统保护提供了关键数据，中国。
The Qinghai-Tibet Plateau is sensitive to climate change, with ecosystems that are important with respect to scientific research. Here high-throughput DNA pyrosequencing was used to assess bacterial diversity within different alpine grassland ecosystems of the Qinghai-Tibet Plateau, China. In total, 34,759 sequences were obtained for the three ecosystems––alpine cold swamp meadow (ASM), alpine cold meadow (AM), alpine sandy grassland (ASG), and 31 phyla and a small number of unclassified bacteria were detected. The bacterial community structures were different for each alpine grassland ecosystem. The Proteobacteria and Acidobacteria were the predominant phyla in all three ecosystems. Besides this, Actinobacteria and Chloroflexi were abundant in ASM, Bacteroidetes, Gemmatimonadetes and Verrucomicrobia were abundant in AM, and Actinobacteria were abundant in ASG. In addition, the functional bacterial genera also differed with each alpine grassland ecosystem. The ASM contained more nitrifying bacteria, methane-oxidizing bacteria and sulfur- and sulfate-reducing bacteria, whereas the ASG ecosystem contained more nitrogen-fixing bacteria. Pyrosequencing provided a greater insight into bacterial diversity within different alpine grassland ecosystems than previously possible, and gave key data for the involvement of bacteria in the protection of alpine grassland ecosystems of the Qinghai-Tibet Plateau, China.
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影响因子：
4.4
作者：
Lipson, DA;Schmidt, SK
通讯作者：
Schmidt, SK
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.4
作者：
Oakley, Brian B.;Fiedler, Tina L.;Fredricks, David N.
通讯作者：
Fredricks, David N.
影响因子：
5.1
作者：
Costello, Elizabeth K.;Schmidt, Steven K.
通讯作者：
Schmidt, Steven K.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM