Expression of transforming growth factor-β in the rat ventral prostate during castration-induced programmed cell death
Expression of transforming growth factor-β in the rat ventral prostate during castration-induced programmed cell death
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去势诱导的程序性细胞死亡过程中大鼠腹侧前列腺中转化生长因子-β的表达
DOI：
--
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Isaacs
中科院分区：
文献类型：
--
作者：
N. Kyprianou;J. Isaacs
去势诱导的雄激素剥夺导致大鼠腹侧前列腺中雄激素依赖性前列腺上皮细胞程序性死亡的激活。为了鉴定这种程序性细胞死亡的潜在介质，在去势诱导雄激素撤退后，研究了大鼠腹侧前列腺中转化生长因子-β（TGFβ）的表达。通过 Northern 印迹分析确定 TGF β mRNA 的稳态水平，并与前列腺素 C3（腹侧前列腺的主要雄激素依赖性分泌蛋白）的 mRNA 水平以及 TRPM-2（一种在去势诱导的前列腺细胞死亡过程中特异性表达的基因）的 mRNA 水平进行比较。在去势后的第一天内，腹侧前列腺中的 TGF β mRNA 水平急剧增加（约 10 倍），到去势后 4 天，TGF β mRNA 表达达到最大（约增加 40 倍），此时雄激素依赖性 C3 分泌蛋白 mRNA 转录物已减少至不可检测的水平。对4天去势的大鼠施用雄激素导致TGFβmRNA显着降低至与其在完整对照动物中获得的组成型表达相当的水平，表明大鼠腹侧前列腺中TGFβ的表达处于负雄激素调节之下。编码 TRPM-2 的转录水平最初在去势后第一天内增加了 10 倍，到去势后第 4 天出现了戏剧性的增加（约 50 倍），这与雄激素依赖性前列腺上皮细胞的最大细胞死亡率相关。（摘要截断为 250 字）
Castration-induced androgen deprivation leads to the activation of the programmed death of the androgen-dependent prostatic epithelial cells in the rat ventral prostate. In order to identify potential mediators of this programmed cell death, the expression of transforming growth factor-beta (TGF beta) in the rat ventral prostate was studied, after castration induced-androgen withdrawal. Steady state levels of TGF beta mRNA were determined by Northern blot analysis and compared with mRNA levels for prostatein C3, the major androgen-dependent secretory protein of ventral prostate and also with mRNA levels for TRPM-2, a gene that is specifically expressed during castration induced prostatic cell death. Within the first day after castration there was a dramatic increase in the levels of TGF beta mRNA in the ventral prostate (approximately 10-fold) and by 4 days after castration TGF beta mRNA was maximally expressed (approximately 40-fold increase), by which time the androgen-dependent C3 secretory protein mRNA transcripts have diminished to undetectable levels. Androgen administration to 4-day castrated rats led to a marked decrease in TGF beta mRNA to a level comparable to its constitutive expression obtained in the intact control animals, indicating that expression of TGF beta in the rat ventral prostate is under negative androgenic regulation. The transcript levels encoding TRPM-2 initially increased 10-fold within the first day after castration and by day 4 post castration there was a dramatic increase (approximately 50-fold) which correlated well with the maximal rate of cell death of the androgen-dependent prostatic epithelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)
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DOI：
10.1016/b978-0-12-571139-5.50018-5
发表时间：
1983
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Gerald R. Cunha;L. W. Chung;John M. Shannon;O. Taguchi;H. Fujii
通讯作者：
Gerald R. Cunha;L. W. Chung;John M. Shannon;O. Taguchi;H. Fujii
DOI：
10.1073/pnas.84.17.6020
发表时间：
1987-09-01
影响因子：
11.1
作者：
ASSOIAN, RK;FLEURDELYS, BE;SPORN, MB
通讯作者：
SPORN, MB
影响因子：
4.1
作者：
BETTUZZI, S;HIIPAKKA, RA;LIAO, SS
通讯作者：
LIAO, SS
影响因子：
4.8
作者：
KYPRIANOU, N;ISAACS, JT
通讯作者：
ISAACS, JT
通过 cDNA 克隆和序列分析将谷胱甘肽 S-转移酶 Yb1 mRNA 鉴定为雄激素抑制 mRNA。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chang,CS;Saltzman,AG;Sorensen,NS;Hiipakka,RA;Liao,SS
通讯作者：
Liao,SS