Short-term exposure to ethanol induces transcriptional changes in nontumorigenic breast cells.
Short-term exposure to ethanol induces transcriptional changes in nontumorigenic breast cells.
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DOI：
10.1002/2211-5463.13693
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发表时间：
2023-10
期刊：
影响因子：
2.6
通讯作者：
Kugel JF
中科院分区：
文献类型：
--
作者：
Miller GM;Brant TS;Goodrich JA;Kugel JF
乳腺癌是女性与癌症相关的主要原因。对于最终了解酒精和乳腺癌的关系，我们研究了诱发的转录变化，以响应于中等水平的细胞暴露于中等水平的酒精。 。只有MCF10A细胞系在响应乙醇治疗的新转录RNA上暴露了统计学上的显着变化，这表明某些乙醇中调节的基因对酒精治疗的剂量敏感，而其他基因则不是基因本体学和生物化学途径。
研究已经建立了饮酒和乳腺癌之间的联系，但是，这种关系的分子基础使用肿瘤乳房和非独居的乳腺细胞进行了了解。
Breast cancer is a leading cause of cancer‐related deaths in women. Many genetic and behavioral risk factors can contribute to the initiation and progression of breast cancer, one being alcohol consumption. Numerous epidemiological studies have established a positive correlation between alcohol consumption and breast cancer; however, the molecular basis for this link remains ill defined. Elucidating ethanol‐induced changes to global transcriptional programming in breast cells is important to ultimately understand how alcohol and breast cancer are connected mechanistically. We investigated induced transcriptional changes in response to a short cellular exposure to moderate levels of alcohol. We treated the nontumorigenic breast cell line MCF10A and the tumorigenic breast cell lines MDA‐MB‐231 and MCF7, with ethanol for 6 h, and then captured the changes to ongoing transcription using 4‐thiouridine metabolic labeling followed by deep sequencing. Only the MCF10A cell line exhibited statistically significant changes in newly transcribed RNA in response to ethanol treatment. Further experiments revealed that some ethanol‐upregulated genes are sensitive to the dose of alcohol treatment, while others are not. Gene Ontology and biochemical pathway analyses revealed that ethanol‐upregulated genes in MCF10A cells are enriched in biological functions that could contribute to cancer development. Studies have established a link between alcohol consumption and breast cancer; however, the molecular basis for this relationship is poorly understood. Using tumorigenic and nontumorigenic breast cells, we evaluated changes in transcription induced by a short exposure to moderate levels of alcohol. Alcohol‐upregulated genes in nontumorigenic cells are enriched in biological functions that could contribute to cancer development.
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影响因子：
5.2
作者：
Gelfand R;Vernet D;Bruhn K;Vadgama J;Gonzalez-Cadavid NF
通讯作者：
Gonzalez-Cadavid NF
使用有效和可逆的共价化学跟踪不同的RNA群体。
影响因子：
16
作者：
Duffy EE;Rutenberg-Schoenberg M;Stark CD;Kitchen RR;Gerstein MB;Simon MD
通讯作者：
Simon MD
影响因子：
2.3
作者：
AHLUWALIA, BS;WESTNEY, LS;RAJGURU, SU
通讯作者：
RAJGURU, SU
TMEM158的过表达有助于卵巢癌发生。
DOI：
10.1186/s13046-015-0193-y
发表时间：
2015-08-04
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Cheng Z;Guo J;Chen L;Luo N;Yang W;Qu X
通讯作者：
Qu X
影响因子：
14.9
作者：
Huang DW;Sherman BT;Tan Q;Kir J;Liu D;Bryant D;Guo Y;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA