Transcriptome analysis of axillary buds in low phosphorus stress and functional analysis of TaWRKY74s in wheat.
Transcriptome analysis of axillary buds in low phosphorus stress and functional analysis of TaWRKY74s in wheat.
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低磷胁迫下小麦腋芽的转录组分析及TaWRKY74s的功能分析
DOI：
10.1186/s12870-023-04695-w
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发表时间：
2024-01-02
影响因子：
5.3
通讯作者：
Wang, Fang
中科院分区：
文献类型：
--
作者：
Li, Xue-zheng;Zhang, Xiao-tong;Bie, Xiao-min;Zhang, Jing;Jiang, Deng-ji;Tang, Heng;Wang, Fang
小麦是世界主要粮食作物之一，分蘖数是影响小麦产量的关键因素。磷是小麦分蘖发育的必需元素。然而，由于土壤中磷含量的下降，磷肥的使用量不断增加，同时也带来了土壤和水体污染的风险。因此，鉴定小麦耐低磷基因并利用其进行小麦抗逆育种具有重要意义。我们对小麦品种科农199(KN199)进行了低磷胁迫，观察到分蘖数减少。通过转录组分析，我们发现了1651个低磷胁迫后上调的基因和827个下调的基因。差异表达基因被发现在与磷、激素信号转导和离子跨膜运输相关的酶活性调节方面表现出丰富的表达。此外，转录因子分析表明，TaWRKY74s在耐低磷中起重要作用。TaWRKY74有三个等位基因：TaWRKY74-A、TaWRKY74-B和TaWRKY74-D，它们都属于WRKY家族，具有保守的WRKYGQK基序。这些蛋白定位于细胞核，在腋生分生组织、顶端分生组织、幼叶、叶原基和小穗原基中均有表达。进化树分析表明，水稻TaWRKY74S与OsWRKY74S亲缘关系密切。此外，在正常条件下，转TaWRKY74S-RNAi基因植株的分蘖数明显少于野生型植株。此外，在低磷胁迫下，转RNAi基因植株的分蘖数也显著低于野生型植株，且降低幅度增大。这表明TaWRKY74S与磷反应有关，并能影响小麦的分蘖数。研究结果表明，TaWRKY74S是小麦响应低磷胁迫的关键基因，可能通过脱落酸(ABA)和生长素信号转导途径调控小麦分蘖数。本研究为进一步研究TaWRKY74S在低磷环境下的抗逆机理奠定了基础，对小麦抗逆性育种具有重要意义。网上版载有补充材料，可在10.1186/s12870023-04695-w查阅。
Wheat is one of the main grain crops in the world, and the tiller number is a key factor affecting the yield of wheat. Phosphorus is an essential element for tiller development in wheat. However, due to decreasing phosphorus content in soil, there has been increasing use of phosphorus fertilizer, while imposing risk of soil and water pollution. Hence, it is important to identify low phosphorus tolerance genes and utilize them for stress resistance breeding in wheat. We subjected the wheat variety Kenong 199 (KN199) to low phosphorus stress and observed a reduced tiller number. Using transcriptome analysis, we identified 1651 upregulated genes and 827 downregulated of genes after low phosphorus stress. The differentially expressed genes were found to be enriched in the enzyme activity regulation related to phosphorus, hormone signal transduction, and ion transmembrane transport. Furthermore, the transcription factor analysis revealed that TaWRKY74s were important for low phosphorus tolerance. TaWRKY74s have three alleles: TaWRKY74-A, TaWRKY74-B, and TaWRKY74-D, and they all belong to the WRKY family with conserved WRKYGQK motifs. These proteins were found to be located in the nucleus, and they were expressed in axillary meristem, shoot apical meristem(SAM), young leaves, leaf primordium, and spikelet primordium. The evolutionary tree showed that TaWRKY74s were closely related to OsWRKY74s in rice. Moreover, TaWRKY74s-RNAi transgenic plants displayed significantly fewer tillers compared to wild-type plants under normal conditions. Additionally, the tiller numebr of the RNAi transgenic plants was also significantly lower than that of the wild-type plants under low-phosphorus stress, and increased the decrease amplitude. This suggestd that TaWRKY74s are related to phosphorus response and can affect the tiller number of wheat. The results of this research showed that TaWRKY74s were key genes in wheat response to low phosphorus stress, which might regulate wheat tiller number through abscisic acid (ABA) and auxin signal transduction pathways. This research lays the foundation for further investigating the mechanism of TaWRKY74s in the low phosphorus environments and is significant for wheat stress resistance breeding. The online version contains supplementary material available at 10.1186/s12870-023-04695-w.
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影响因子：
5.2
作者：
Jung, Harin;Lee, Dong-Keun;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon
DOI：
10.3390/plants11162119
发表时间：
2022-08-15
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
DOI：
10.3390/plants9111515
发表时间：
2020-11-08
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Li W;Pang S;Lu Z;Jin B
通讯作者：
Jin B
影响因子：
4.6
作者：
Liu L;Zheng X;Wei X;Kai Z;Xu Y
通讯作者：
Xu Y
影响因子：
16.6
作者：
Wang, Huan;Zou, Shenghao;Tang, Dingzhong
通讯作者：
Tang, Dingzhong