Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model.
Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model.
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DOI：
10.1371/journal.pone.0160667
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kramer A
中科院分区：
文献类型：
--
作者：
Kluge S;Bekeschus S;Bender C;Benkhai H;Sckell A;Below H;Stope MB;Kramer A
用于生物医学应用的所谓冷物理等离子体产生活性氧和活性氮，后者在高浓度时可引发DNA损伤。因此，我们评估了经认证的常压氩等离子体射流（kINPen MED）及其前身模型（kINPen 09）的致突变风险。用kINPen 09（1.5、2.0或2.5分钟）或kINPen MED（3、4、5或10分钟）对受精卵内蛋膜进行单次处理。孵育3天后，进行血涂片（全光学mayr - gr<s:1> nwald- giemsa染色），并评估每个鸡蛋1000个红细胞是否存在多色和正色核染色，以及核畸变和双核细胞（鸡蛋微核诱导试验，HET-MN）。同时，记录了胚胎死亡率。每个实验均设阳性对照（环磷酰胺和甲氨蝶呤）和阴性对照（氯化钠溶液、氩气）。另外，用抗坏血酸氧化法测定治疗后血浆的抗氧化潜能。对于两种血浆源，没有证据表明存在遗传毒性，尽管在最长的血浆暴露时间为10分钟时，胚胎死亡率超过40%。暴露于低温血浆后，鸡蛋血浆中的抗氧化能力没有立即（p = 0.32）或1小时（p = 0.19）显著降低。使用kINPen MED的最长血浆治疗时间是临床治疗慢性伤口推荐限度的5-10倍。我们没有发现使用kINPen 09或kINPen MED的任何血浆治疗时间的致突变作用。目前研究提供的数据似乎证实缺乏基因毒性潜力，这表明这些氩等离子射流的兽医或临床应用不会造成致突变风险。
So-called cold physical plasmas for biomedical applications generate reactive oxygen and nitrogen species and the latter can trigger DNA damage at high concentrations. Therefore, the mutagenic risks of a certified atmospheric pressure argon plasma jet (kINPen MED) and its predecessor model (kINPen 09) were assessed. Inner egg membranes of fertilized chicken eggs received a single treatment with either the kINPen 09 (1.5, 2.0, or 2.5 min) or the kINPen MED (3, 4, 5, or 10 min). After three days of incubation, blood smears (panoptic May-Grünwald-Giemsa stain) were performed, and 1000 erythrocytes per egg were evaluated for the presence of polychromatic and normochromic nuclear staining as well as nuclear aberrations and binucleated cells (hen’s egg test for micronuclei induction, HET-MN). At the same time, the embryo mortality was documented. For each experiment, positive controls (cyclophosphamide and methotrexate) and negative controls (NaCl-solution, argon gas) were included. Additionally, the antioxidant potential of the blood plasma was assessed by ascorbic acid oxidation assay after treatment. For both plasma sources, there was no evidence of genotoxicity, although at the longest plasma exposure time of 10 min the mortality of the embryos exceeded 40%. The antioxidant potential in the egg’s blood plasma was not significantly reduced immediately (p = 0.32) or 1 h (p = 0.19) post exposure to cold plasma. The longest plasma treatment time with the kINPen MED was 5–10 fold above the recommended limit for treatment of chronic wounds in clinics. We did not find mutagenic effects for any plasma treatment time using the either kINPen 09 or kINPen MED. The data provided with the current study seem to confirm the lack of a genotoxic potential suggesting that a veterinary or clinical application of these argon plasma jets does not pose mutagenic risks.
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影响因子：
1.5
作者：
Bekeschus, Sander;Iseni, Sylvain;Weltmann, Klaus-Dieter
通讯作者：
Weltmann, Klaus-Dieter
影响因子：
--
作者：
Bekeschus, Sander;Schmidt, Anke;von Woedtke, Thomas
通讯作者：
von Woedtke, Thomas
影响因子：
3.2
作者：
Bender, Claudia;Partecke, Lars-Ivo;Huebner, Nils-Olaf
通讯作者：
Huebner, Nils-Olaf
DOI：
10.1098/rstb.1973.0050
发表时间：
1973-01-01
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BRUNS, GAP;INGRAM, VM
通讯作者：
INGRAM, VM
DOI：
10.1073/pnas.80.11.3372
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HAMILTON, JW;DENISON, MS;BLOOM, SE
通讯作者：
BLOOM, SE