The genetic determinants of 4-chloro-2-nitrophenol degradation in Cupriavidus sp. strain NyZ417
The genetic determinants of 4-chloro-2-nitrophenol degradation in Cupriavidus sp. strain NyZ417
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Cupriavidus sp. 4-氯-2-硝基苯酚降解的遗传决定因素。
DOI：
10.1016/j.ibiod.2020.105170
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发表时间：
2021-03
影响因子：
4.8
通讯作者：
Ning-Yi Zhou
中科院分区：
文献类型：
--
作者：
Shi-Kai Deng;Ning-Yi Zhou
4-氯-2-硝基苯酚（4C 2NP）是一种典型的氯代硝基芳香族污染物，具有致突变性和致癌性，其环境归趋受到广泛关注。到目前为止，没有基因或酶负责4C 2NP生物降解已阐明。从我国某污水处理厂的活性污泥中分离到一株4C 2NP降解菌NyZ 417，经16 S rRNA基因序列分析，该菌株属于贪铜菌属Cupriavidussp.菌株NyZ 417以4C 2NP为唯一氮源，在外加碳源的条件下生长。菌株NyZ 417对4C 2NP的降解是通过氧化途径进行的，并且是由底物本身诱导的。为了阐明该菌株降解4C 2NP的分子机制，从其全基因组中检索到一个编码单加氧酶的基因pANyZ 417，证实其参与了4C 2NP的生物转化。表达OnpANyZ 417的静息细胞完全耗尽4C 2NP，化学计量形成4-氯儿茶酚。本研究不仅阐明了4C 2NP降解的功能基因，而且为设计环境中4C 2NP残留的清除方法奠定了坚实的基础。
4-chloro-2-nitrophenol (4C2NP) is a typical chlorinated nitroaromatic pollutant and its environmental fate is of great concern due to its mutagenic and carcinogenic properties. So far, neither genes nor enzymes responsible for 4C2NP biodegradation have been elucidated. Here, a new 4C2NP degrader NyZ417 was isolated from the activated sludge collected from a wastewater treatment plant of China and identified as a member withinCupriavidusgenus according to its 16S rRNA gene sequence analysis.Cupriavidussp. strain NyZ417 utilized 4C2NP as the sole of nitrogen source for its growth in the presence of additional carbon source. The degradation of 4C2NP by strain NyZ417 was confirmed via an oxidative pathway and induced by substrate itself. For the elucidation of molecular mechanism of 4C2NP degradation in this newly isolated degrader, a monooxygenase encoding geneonpANyZ417retrieved from its complete genome was confirmed to be involved in 4C2NP biotransformation. Resting cells expressing OnpANyZ417depleted 4C2NP completely with stoichiometric formation of 4-chlorocatechol. This study not only elucidated a functional gene for 4C2NP degradation but also would lay a solid foundation for designing approaches for clean-up of 4C2NP residues in the environment.
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DOI：
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发表时间：
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期刊：
International Biodeterioration & Biodegradation
影响因子：
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