A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula.
A combined transcriptome - miRNAome approach revealed that a kinesin gene is differentially targeted by a novel miRNA in an apomictic genotype of Eragrostis curvula.
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DOI：
10.3389/fpls.2022.1012682
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
弯画眉草（Eragrostis curvula [Shrad.] Nees）是一种多年生草本植物，通常生长在半干旱地区，对干旱条件和桑迪土壤具有良好的适应性。这种多态复合体包括有性和无融合生殖细胞型，具有不同的倍性水平（2x-8x）。已知二倍体是有性的，而大多数多倍体是兼性无融合生殖，也有报道完全无融合生殖。多年来，植物育种研究的重点是实现无融合生殖到农业相关物种的渐渗，但是，鉴于性状的复杂性，仍然需要更深入地了解无融合生殖调控机制的分子基础。无融合生殖被认为与性途径的沉默或中断有关，研究表明它受到表观遗传机制的影响。在先前的研究中，我们使用两种不同的E.弯孢表型本研究通过对有性OTA-S、兼性Don Walter和专性无融合生殖Tanganyika的cDNA和sRNA文库的查询，寻找与生殖方式相关基因的miRNA发现和miRNA表达调控。这允许表征七种miRNA并验证其miRNA-靶标相互作用。有趣的是，发现驱动蛋白基因在无融合生殖品种Tanganyika中被抑制，被发现在该基因型中过表达的新型miRNA靶向，暗示参与生殖模式表达。我们的工作为无融合生殖途径的表观遗传调控的贡献提供了额外的证据。
Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass typically established in semi-arid regions, with good adaptability to dry conditions and sandy soils. This polymorphic complex includes both sexual and apomictic cytotypes, with different ploidy levels (2x-8x). Diploids are known to be sexual, while most polyploids are facultative apomicts, and full apomicts have also been reported. Plant breeding studies throughout the years have focused on achieving the introgression of apomixis into species of agricultural relevance, but, given the complexity of the trait, a deeper understanding of the molecular basis of regulatory mechanisms of apomixis is still required. Apomixis is thought to be associated with silencing or disruption of the sexual pathway, and studies have shown it is influenced by epigenetic mechanisms. In a previous study, we explored the role of miRNA-mRNA interactions using two contrasting E. curvula phenotypes. Here, the sexual OTA-S, the facultative Don Walter and the obligate apomictic Tanganyika cDNA and sRNA libraries were inquired, searching for miRNA discovery and miRNA expression regulation of genes related to the reproductive mode. This allowed for the characterization of seven miRNAs and the validation of their miRNA-target interactions. Interestingly, a kinesin gene was found to be repressed in the apomictic cultivar Tanganyika, targeted by a novel miRNA that was found to be overexpressed in this genotype, suggestive of an involvement in the reproductive mode expression. Our work provided additional evidence of the contribution of the epigenetic regulation of the apomictic pathway.
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影响因子：
6.9
作者：
Frey N;Klotz J;Nick P
通讯作者：
Nick P
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1038/nrm4085
发表时间：
2015-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Borges F;Martienssen RA
通讯作者：
Martienssen RA
影响因子：
4.6
作者：
Zappacosta DC;Ochogavía AC;Rodrigo JM;Romero JR;Meier MS;Garbus I;Pessino SC;Echenique VC
通讯作者：
Echenique VC
影响因子：
2.5
作者：
Brukhin V;Albertini E
通讯作者：
Albertini E