A kinesin with calponin-homology domain is involved in premitotic nuclear migration.
A kinesin with calponin-homology domain is involved in premitotic nuclear migration.
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DOI：
10.1093/jxb/erq164
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发表时间：
2010-07
影响因子：
6.9
通讯作者：
Nick P
中科院分区：
文献类型：
--
作者：
Frey N;Klotz J;Nick P
微管和肌动蛋白微丝之间的相互作用和串扰对于植物生长和发育过程中的许多过程都很重要，包括细胞伸长和组织扩张的控制，但对这种相互作用的分子组成知之甚少。具有钙调蛋白同源结构域（KCH）的植物驱动蛋白最近被鉴定出来，并与微管-微丝交联的假定作用相关。水稻KCH成员OsKCH 1的假定的生物学作用在这里解决了一方面使用Tos 17 KCH 1敲除突变体的组合方法，和在烟草BY-2细胞中产生的KCH 1过表达系。结果表明，OsKCH 1在水稻中以发育和组织特异性的方式表达，并且报道了由于敲低和过表达而引起的拮抗细胞伸长和分裂表型。此外，OsKCH 1在细胞周期中的动态再分配的描述，它表明，KCH过表达延迟核定位和有丝分裂在BY-2细胞。这些研究结果进行了讨论相对于一个假定的作用KCHs作为肌动蛋白丝和微管之间的连接器在核定位。
Interaction and cross-talk between microtubules and actin microfilaments are important for numerous processes during plant growth and development, including the control of cell elongation and tissue expansion, but little is known about the molecular components of this interaction. Plant kinesins with the calponin-homology domain (KCH) were recently identified and associated with a putative role in microtubule-microfilament cross-linking. The putative biological role of the rice KCH member OsKCH1 is addressed here using a combined approach with Tos17 kch1 knock-out mutants on the one hand, and a KCH1 overexpression line generated in tobacco BY-2 cells. It is shown that OsKCH1 is expressed in a development and tissue-specific manner in rice and antagonistic cell elongation and division phenotypes as a result of knock-down and overexpression are reported. Further, the dynamic repartitioning of OsKCH1 during the cell cycle is described and it is demonstrated that KCH overexpression delays nuclear positioning and mitosis in BY-2 cells. These findings are discussed with respect to a putative role of KCHs as linkers between actin filaments and microtubules during nuclear positioning.
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影响因子：
10.5
作者：
Chen, JG;Ullah, H;Jones, AM
通讯作者：
Jones, AM
影响因子：
4.3
作者：
BASKIN, TI;BIVENS, NJ
通讯作者：
BIVENS, NJ
影响因子：
4.3
作者：
McClinton, RS;Sung, ZR
通讯作者：
Sung, ZR
影响因子：
4.8
作者：
Blancaflor, EB
通讯作者：
Blancaflor, EB
DOI：
10.1023/a:1009832822096
发表时间：
1999-01-01
期刊：
Methods in Cell Science
影响因子：
--
作者：
Nagata, Toshiyuki;Kumagai, Fumi
通讯作者：
Kumagai, Fumi