Transcriptomic analysis reveals pronounced changes in gene expression due to sub-lethal pyrethroid exposure and ageing in insecticide resistance Anopheles coluzzii.
Transcriptomic analysis reveals pronounced changes in gene expression due to sub-lethal pyrethroid exposure and ageing in insecticide resistance Anopheles coluzzii.
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转录组学分析揭示了由于亚致死拟除虫菊酯暴露和杀虫剂抗性的老化引起的基因表达的显著变化。
DOI：
10.1186/s12864-021-07646-7
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发表时间：
2021-05-10
期刊：
影响因子：
4.4
通讯作者：
Ranson H
中科院分区：
文献类型：
--
作者：
Ingham VA;Brown F;Ranson H
疟疾控制在很大程度上依赖于使用针对并杀死成年雌性疟蚊媒介的杀虫剂。大量使用拟除虫菊酯类杀虫剂已导致蚊子种群普遍产生抗药性。在非洲的一些地区，拟除虫菊酯类杀虫剂抗药性的强度意味着蚊子可以多次接触用这类杀虫剂处理过的蚊帐，而死亡率最低。此外，衰老和死亡周期都显示对抗性表型有很大影响。总之，这些特征可能影响病媒生物学的其他方面，控制蚊子的病媒能力或适应性。本研究表明，原来自布基纳法索的高抗性科鲁兹按蚊亚致死暴露于拟除虫菊酯类杀虫剂溴氰菊酯后，转录物表达发生了巨大而持续的变化。我们在数据中确定了五种清晰的模式，显示了与DNA修复、呼吸、翻译、行为和氧化还原酶过程相关的转录本变化。此外，我们强调了先前与杀虫剂抗性相关的解毒家族转录本的差异调控，以及氧化磷酸化途径的明确下调，这两者都表明了暴露后代谢的变化。最后，我们发现衰老和死亡循环对已知的杀虫剂抗性相关转录本都有主要影响。亚致死拟除虫菊酯暴露、衰老和死亡周期导致主要疟疾媒介科鲁兹按蚊转录组发生大规模变化。我们的数据有力地支持了进一步的表型研究，即转录变化（如氧化磷酸化途径的表达减少或拟除虫菊酯诱导的氧化还原状态的变化）如何影响蚊子的关键特征，如媒介能力和生活史特征。在线版本包含补充材料，可在10.1186/s12864-021-07646-7获得。
Malaria control is heavily reliant on the use of insecticides that target and kill the adult female Anopheline vector. The intensive use of insecticides of the pyrethroid class has led to widespread resistance in mosquito populations. The intensity of pyrethroid resistance in some settings in Africa means mosquitoes can contact bednets treated with this insecticide class multiple times with minimal mortality effects. Furthermore, both ageing and diel cycle have been shown to have large impacts on the resistance phenotype. Together, these traits may affect other aspects of vector biology controlling the vectorial capacity or fitness of the mosquito. Here we show that sublethal exposure of a highly resistant Anopheles coluzzii population originally from Burkina Faso to the pyrethroid deltamethrin results in large and sustained changes to transcript expression. We identify five clear patterns in the data showing changes to transcripts relating to: DNA repair, respiration, translation, behaviour and oxioreductase processes. Further, we highlight differential regulation of transcripts from detoxification families previously linked with insecticide resistance, in addition to clear down-regulation of the oxidative phosphorylation pathway both indicative of changes in metabolism post-exposure. Finally, we show that both ageing and diel cycle have major effects on known insecticide resistance related transcripts. Sub-lethal pyrethroid exposure, ageing and the diel cycle results in large-scale changes in the transcriptome of the major malaria vector Anopheles coluzzii. Our data strongly supports further phenotypic studies on how transcriptional changes such as reduced expression of the oxidative phosphorylation pathway or pyrethroid induced changes to redox state might impact key mosquito traits, such as vectorial capacity and life history traits. The online version contains supplementary material available at 10.1186/s12864-021-07646-7.
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影响因子：
3.8
作者：
Ahn, Seung-Joon;Vogel, Heiko;Heckel, David G.
通讯作者：
Heckel, David G.
影响因子：
4.1
作者：
Hu, Fei;Dou, Wei;Wang, Jin-Jun
通讯作者：
Wang, Jin-Jun
DOI：
10.4269/ajtmh.2003.68.121
发表时间：
2003-04-01
影响因子：
3.3
作者：
Hawley, WA;Phillips-Howard, PA;Hightower, AW
通讯作者：
Hightower, AW
影响因子：
3.5
作者：
Elanga-Ndille, Emmanuel;Nouage, Lynda;Wondji, Charles S.
通讯作者：
Wondji, Charles S.
影响因子：
1.9
作者：
Fetoui, Hamadi;Feki, Ameni;Gdoura, Radhouane
通讯作者：
Gdoura, Radhouane